Intracellular free calcium regulates the onset of the respiratory burst of human neutrophils activated by phorbol myristate acetate | |
Hu, T. H. ; Bei, L. ; Qian, Z. M. ; Shen, X. ; Hu TH(胡天惠) | |
1999 | |
关键词 | respiratory burst neutrophil intracellular calcium protein kinase C thapsigargin signal transduction |
英文摘要 | Thapsigargin was used to study the regulation of different static calcium level ([Ca2+](i)) on the respiratory burst of human neutrophils stimulated with phorbol myristate acetate (PMA). The result showed that the onset time of the respiratory burst was obviously reduced by elevation of static [Ca2+](i) but is still much longer than that stimulated with N-formylmethionylleucylphenylalanine (fMLP). To find the reason, the onset times of the respiratory burst stimulated with fMLP, 1,2-dioctanoyl-sn-glycerol (DiC8), and PMA were determined at different static [Ca2+](i). It turns out that although DiC8 was unable to induce the respiratory burst at low [Ca2+](i), the onset time of DiC8-stimulated response at high [Ca2+](i) was almost the same as that stimulated with fMLP. The study revealed that the fast onset of the fMLP-stimulated respiratory burst in comparison with PMA-stimulated response is not only due to the transient rise of [Ca2+](i), but is also due to the higher efficiency of diacylglycerol (DAG) in activating protein kinase c (PKC). The determining step in governing the onset of a respiratory burst is the activation of PKC. CELL SIGNAL 11;5:355-360, 1999. (C) 1999 Elsevier Science Inc. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/65455] ![]() |
专题 | 生命科学-已发表论文 |
推荐引用方式 GB/T 7714 | Hu, T. H.,Bei, L.,Qian, Z. M.,et al. Intracellular free calcium regulates the onset of the respiratory burst of human neutrophils activated by phorbol myristate acetate[J],1999. |
APA | Hu, T. H.,Bei, L.,Qian, Z. M.,Shen, X.,&胡天惠.(1999).Intracellular free calcium regulates the onset of the respiratory burst of human neutrophils activated by phorbol myristate acetate.. |
MLA | Hu, T. H.,et al."Intracellular free calcium regulates the onset of the respiratory burst of human neutrophils activated by phorbol myristate acetate".(1999). |
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