QQ客服 官方微博 反馈留言

	CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue
	Shi, Y. ; Reitmaier, B. ; Regenbogen, J. ; Slowey, R. M. ; Opalenik, S. R. ; Wolf, E. ; Goppelt, A. ; Davidson, J. M. ; Shi Y(石艳)
	2005
关键词	ENDOTHELIAL-CELLS TRANSCRIPTION FACTOR TRANSGENIC MICE GENE-THERAPY GROWTH EXPRESSION EGR-1 PROMOTER IDENTIFICATION INDUCTION
英文摘要	Cardiac ankyrin repeat protein (CARP) was identified by subtractive hybridization as one of a group of genes that are rapidly modulated by acute wounding of mouse skin. Quantitative RT-PCR showed that CARP was strongly induced during the first day after wounding (157.1-fold), and the high level persisted for up to 14 days. Immunohistochemistry and in situ hybridization revealed that CARP was expressed in skeletal muscle, vessel wall, hair follicle, inflammatory cells, and epidermis in the wound area. To examine the effects of CARP on wound healing, we developed an adenoviral CARP vector to treat subcutaneously implanted sponges. in either rats or Flk-1(LacZ) knock-in mice. Four days after infection, CARP-infected sponges in rats showed a remarkable increase in the vascular component in granulation tissue as compared to Ad-LacZ controls. This result was confirmed by CD34 immunostaining. By 7 days post-infection of sponge implants in Flk-1(LacZ) knock-in mice, granulation tissue showed many more LacZ-positive cells in Ad-CARP-infected sponges than in virus controls. Ad-CARP treatment also induced neovascularization and increased blood perfusion in rabbit excisional wounds in and ischemic rat wounds. These findings indicate that CARP could play a unique role in therapeutic angiogenesis during wound healing.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65434]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Shi, Y.,Reitmaier, B.,Regenbogen, J.,et al. CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue[J],2005.
APA	Shi, Y..,Reitmaier, B..,Regenbogen, J..,Slowey, R. M..,Opalenik, S. R..,...&石艳.(2005).CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue..
MLA	Shi, Y.,et al."CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue".(2005).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。