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	Authentication of Chinese crude drug, Gecko, by allele-specific diagnostic PCR
	Liu, Z. Q. ; Wang, Y. Q. ; Zhou, K. Y. ; Han, D. M. ; Yang, X. G. ; Liu, X. H. ; Wang YQ(王义权)
	2001
英文摘要	Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of Gekko gecko were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources, Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65375]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liu, Z. Q.,Wang, Y. Q.,Zhou, K. Y.,et al. Authentication of Chinese crude drug, Gecko, by allele-specific diagnostic PCR[J],2001.
APA	Liu, Z. Q..,Wang, Y. Q..,Zhou, K. Y..,Han, D. M..,Yang, X. G..,...&王义权.(2001).Authentication of Chinese crude drug, Gecko, by allele-specific diagnostic PCR..
MLA	Liu, Z. Q.,et al."Authentication of Chinese crude drug, Gecko, by allele-specific diagnostic PCR".(2001).

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