PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation

CORC > 厦门大学 > 生命科学－已发表论文

	PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
	Ka Ruan ; Fan Ye ; Chenyu Li ; Yih-Cherng Liou ; Sheng-Cai Lin ; Lin SC(林圣彩) ; Shu-Yong Lin
刊名	http://dx.doi.org/10.1371/journal.pone.0049184
	2012-11-14
关键词	CHROMOSOMAL INSTABILITY POLO-LIKE-KINASE-1 PLK1 AMPLIFICATION CANCER REDUPLICATION TUMORIGENESIS DUPLICATION ACTIVATION ANEUPLOIDY LOCALIZES
英文摘要	Abnormal amplification of centrosomes could lead to improper chromosome segregation and aneuploidy and is implicated in cancer development. Here, we demonstrate that Axin, a scaffolding protein in Wnt signaling, is phosphorylated by PLK1 during mitosis. Phosphorylation of Axin Ser-157 by PLK1 abolished Axin association with gamma-tubulin, while substitution of Ser-157 with alanine exhibited sustained interaction with gamma-tubulin. In addition, overexpression of Axin-S157A significantly increased the number of cells with multi-centrosomes. These results suggest that the phosphorylation status of Axin, mediated by PLK1, dynamically regulates its association with gamma-tubulin and centrosome formation and segregation.; The work was supported by 111 Project of China (http://www.moe.edu.cn/),#B06016, and by grants from the Natural Science Foundation of China (http://www.nsfc.gov.cn/),#30921005,#31000621 and #31130016.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65321]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ka Ruan,Fan Ye,Chenyu Li,et al. PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation[J]. http://dx.doi.org/10.1371/journal.pone.0049184,2012.
APA	Ka Ruan.,Fan Ye.,Chenyu Li.,Yih-Cherng Liou.,Sheng-Cai Lin.,...&Shu-Yong Lin.(2012).PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation.http://dx.doi.org/10.1371/journal.pone.0049184.
MLA	Ka Ruan,et al."PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation".http://dx.doi.org/10.1371/journal.pone.0049184 (2012).