Cloning, sequencing and phylogenetic analysis of the small GTPase gene cdc-42 from Ancylostoma caninum

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	Cloning, sequencing and phylogenetic analysis of the small GTPase gene cdc-42 from Ancylostoma caninum
	Yang, Y. R. ; Zheng, J. ; Chen, J. X. ; Yang YR(杨玉荣)
刊名	http://dx.doi.org/10.1016/j.exppara.2012.09.005
	2012-12
关键词	PAR PROTEIN LOCALIZATION CELL-POLARITY C-ELEGANS ENTAMOEBA-HISTOLYTICA SCHISTOSOMA-MANSONI TRYPANOSOMA-CRUZI BACTERIAL TOXINS RHO HOMOLOG KINASE-C CDC42
英文摘要	China National Nature Science Foundation [30972181]; Xiamen Science and Technology grant [3502Z20071077]; New Century Talents Support Program from Xiamen University; CDC-42 is a member of the Rho GTPase subfamily that is involved in many signaling pathways, including mitosis, cell polarity, cell migration and cytoskeleton remodeling. Here, we present the first characterization of a full-length cDNA encoding the small GTPase cdc-42, designated as Accdc-42, isolated from the parasitic nematode Ancylostoma caninum. The encoded protein contains 191 amino acid residues with a predicted molecular weight of 21 kDa and displays a high level of identity with the Rho-family GTPase protein CDC-42. Phylogenetic analysis revealed that Accdc-42 was most closely related to Caenorhabditis briggsae cdc-42. Comparison with selected sequences from the free-living nematode Caenorhabditis elegans, Drosophila melanogaster, Xenopus laevis, Danio rerio, Mus musculus and human genomes showed that Accdc-42 is highly conserved. AcCDC-42 demonstrates the highest identity to CDC-42 from C. briggsae (94.2%), and it also exhibits 91.6% identity to CDC-42 from C. elegans and 91.1% from Brugia malayi. Additionally, the transcript of Accdc-42 was analyzed during the different developmental stages of the worm. Accdc-42 was expressed in the L1/L2 larvae, L3 larvae and female and male adults of A. caninum. (C) 2012 Elsevier Inc. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65256]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Yang, Y. R.,Zheng, J.,Chen, J. X.,et al. Cloning, sequencing and phylogenetic analysis of the small GTPase gene cdc-42 from Ancylostoma caninum[J]. http://dx.doi.org/10.1016/j.exppara.2012.09.005,2012.
APA	Yang, Y. R.,Zheng, J.,Chen, J. X.,&杨玉荣.(2012).Cloning, sequencing and phylogenetic analysis of the small GTPase gene cdc-42 from Ancylostoma caninum.http://dx.doi.org/10.1016/j.exppara.2012.09.005.
MLA	Yang, Y. R.,et al."Cloning, sequencing and phylogenetic analysis of the small GTPase gene cdc-42 from Ancylostoma caninum".http://dx.doi.org/10.1016/j.exppara.2012.09.005 (2012).