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	The Integrative Analysis of microRNA and mRNA Expression in Mouse Uterus under Delayed Implantation and Activation
	Ren-Wei Su ; Wei Lei ; Ji-Long Liu ; Zhi-Rong Zhang ; Bo Jia ; Xu-Hui Feng ; Gang Ren ; Shi-Jun Hu ; Zeng-Ming Yang ; Yang ZM(杨增明)
刊名	http://dx.doi.org/10.1371/journal.pone.0015513
	2010-11-29
关键词	EMBRYO IMPLANTATION GENE-EXPRESSION MATRIX METALLOPROTEINASES PERIIMPLANTATION PERIOD UTERINE RECEPTIVITY SERIAL ANALYSIS MICE DECIDUALIZATION RECEPTOR MIRNAS
英文摘要	National Basic Research Program of China [2011CB944402]; Chinese National Natural Science Foundation [30770244, 30930013]; Background: Delayed implantation is a developmental arrest at the blastocyst stage and a good model for embryo implantation. MicroRNAs (miRNAs) have been shown to be involved in mouse embryo implantation through regulating uterine gene expression. This study was to have an integrative analysis on global miRNA and mRNA expression in mouse uterus under delayed implantation and activation through Illumina sequencing. Methodology/Principal Findings: By deep sequencing and analysis, we found that there are 20 miRNAs up-regulated and 42 miRNAs down-regulated at least 1.2 folds, and 268 genes up-regulated and 295 genes down-regulated at least 2 folds under activation compared to delayed implantation, respectively. Many different forms of editing in mature miRNAs are detected. The percentage of editing at positions 4 and 5 of mature miRNAs is significantly higher under delayed implantation than under activation. Although the number of miR-21 reference sequence under activation is slightly lower than that under delayed implantation, the total level of miR-21 under activation is higher than that under delayed implantation. Six novel miRNAs are predicted and confirmed. The target genes of significantly up-regulated miRNAs under activation are significantly enriched. Conclusions: miRNA and mRNA expression patterns are closely related. The target genes of up-regulated miRNAs are significantly enriched. A high level of editing at positions 4 and 5 of mature miRNAs is detected under delayed implantation than under activation. Our data should be valuable for future study on delayed implantation.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65237]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ren-Wei Su,Wei Lei,Ji-Long Liu,et al. The Integrative Analysis of microRNA and mRNA Expression in Mouse Uterus under Delayed Implantation and Activation[J]. http://dx.doi.org/10.1371/journal.pone.0015513,2010.
APA	Ren-Wei Su.,Wei Lei.,Ji-Long Liu.,Zhi-Rong Zhang.,Bo Jia.,...&杨增明.(2010).The Integrative Analysis of microRNA and mRNA Expression in Mouse Uterus under Delayed Implantation and Activation.http://dx.doi.org/10.1371/journal.pone.0015513.
MLA	Ren-Wei Su,et al."The Integrative Analysis of microRNA and mRNA Expression in Mouse Uterus under Delayed Implantation and Activation".http://dx.doi.org/10.1371/journal.pone.0015513 (2010).

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