QQ客服 官方微博 反馈留言

	Prokaryotical expression of structural and non-structural proteins of hepatitis G virus
	Xia, NS ; Yang, HJ ; Zhang, J ; Lin, CQ ; Wang, YB ; Wang, J ; Zhan, MY ; Ng, MH ; Zhang J(张军)
	2001-10
关键词	NON-A-E HGV INFECTION ANTIBODIES SEQUENCE CLONING LIVER CHINA
英文摘要	AIM To study the epitope distribution of hepatitis G virus (HGV) and to seek for the potential recombinant antigens for the development of HGV diagnositic reagents. METHODS Fourteen clones encompassing HGV gene fragments from core to NS3 and NS5 were constructed using prokaryotic expression vector pRSET and ( or) RGEX, and expressed in E. coli. Western blotting and ELISA were used to detect the immunoreactivity of these recombinant proteins. RESULTS One clone with HGV fragment from core to El (G1), one from E2 (G31), three from NS3 (G6, G61, G7), one from NS5B (GS21) and one chimeric fragment from NS3 and NS5B (G61 - 821) could be expressed well and showed obvious immunoreactivity by Western blotting. One clone with HGV framment from NS5B (GS2) was also well expressed, but could not show immunoreactivity by Western blotting. No obvious expression was found in the, other six clones. All the expressed recombinant proteins were in inclusion body form, except the protein G61 which could be expressed in soluble form. Further purified recombinant proteins G1, G31, G61, G821 and G61 - 821 were detected in indirected ELISA as coating antigen respectively. Only recombinant G1 could still show immunoreactivity, and the other four recombinant proteins failed to react to the HGV antibody positive sera. Western blotting results indicated that the immunoactivity of these four recombinant proteins were lost during purification. CONCLUSION Core to E1, E2, NS3 and NS5 fragment of HGV contain antigenic epitopes, which could be produced in prokaryotically expressed recombinant proteins. A high-yield recombinant protein (G1) located in HGV core to E1 could remain its epitope after purification, which showed the potential that G1 could be used as a coating antigen to develop an ELISA kit for HGV specific antibody diagnosis.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65115]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Xia, NS,Yang, HJ,Zhang, J,et al. Prokaryotical expression of structural and non-structural proteins of hepatitis G virus[J],2001.
APA	Xia, NS.,Yang, HJ.,Zhang, J.,Lin, CQ.,Wang, YB.,...&张军.(2001).Prokaryotical expression of structural and non-structural proteins of hepatitis G virus..
MLA	Xia, NS,et al."Prokaryotical expression of structural and non-structural proteins of hepatitis G virus".(2001).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。