Specific interaction between hnRNP H and HPV16 Li proteins: Implications for late gene auto-regulation enabling rapid viral capsid protein production

CORC > 厦门大学 > 生命科学－已发表论文

	Specific interaction between hnRNP H and HPV16 Li proteins: Implications for late gene auto-regulation enabling rapid viral capsid protein production
	Zheng, Zi-Zheng ; Sun, Yuan-Yuan ; Zhao, Min ; Huang, Hui ; Zhang, Jun ; Xia, Ning-Shao ; Miao, Ji ; Zhao, Qinjian ; Zhang J(张军)
刊名	http://dx.doi.org/10.1016/j.bbrc.2012.12.042
	2013-01-18
关键词	Heterogeneous nuclear ribonucleoprotein (hnRNP) RNA-binding protein Gene regulation Immuno co-precipitation Co-localization of hnRNP H and HPV 16 L1
英文摘要	Heterogeneous nuclear ribonucleoproteins (hnRNPs), including hnRNP H, are RNA-binding proteins that function as splicing factors and are involved in downstream gene regulation. hnRNP H, which binds to G triplet regions in RNA, has been shown to play an important role in regulating the staged expression of late proteins in viral systems. Here, we report that the specific association between hnRNP H and a late viral capsid protein, human papillomavirus (HPV) L1 protein, leads to the suppressed function of hnRNP H in the presence of the L1 protein. The direct interaction between the L1 protein and hnRNP H was demonstrated by complex formation in solution and intracellularly using a variety of biochemical and immunochemical methods, including peptide mapping, specific co-immunoprecipitation and confocal fluorescence microscopy. These results support a working hypothesis that a late viral protein HPV16 L1, which is down regulated by hnRNP H early in the viral life cycle may provide an auto-regulatory positive feedback loop that allows the rapid production of HPV capsid proteins through suppression of the function of hnRNP H at the late stage of the viral life cycle. In this positive feedback loop, the late viral gene products that were down regulated earlier themselves disable their suppressors, and this feedback mechanism could facilitate the rapid production of capsid proteins, allowing staged and efficient viral capsid assembly. (C) 2012 Elsevier Inc. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65108]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zheng, Zi-Zheng,Sun, Yuan-Yuan,Zhao, Min,et al. Specific interaction between hnRNP H and HPV16 Li proteins: Implications for late gene auto-regulation enabling rapid viral capsid protein production[J]. http://dx.doi.org/10.1016/j.bbrc.2012.12.042,2013.
APA	Zheng, Zi-Zheng.,Sun, Yuan-Yuan.,Zhao, Min.,Huang, Hui.,Zhang, Jun.,...&张军.(2013).Specific interaction between hnRNP H and HPV16 Li proteins: Implications for late gene auto-regulation enabling rapid viral capsid protein production.http://dx.doi.org/10.1016/j.bbrc.2012.12.042.
MLA	Zheng, Zi-Zheng,et al."Specific interaction between hnRNP H and HPV16 Li proteins: Implications for late gene auto-regulation enabling rapid viral capsid protein production".http://dx.doi.org/10.1016/j.bbrc.2012.12.042 (2013).