Transcriptome Analysis of Male and Female Sebastiscus marmoratus

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	Transcriptome Analysis of Male and Female Sebastiscus marmoratus
	Lingbin Sun ; Chonggang Wang ; Lixing Huang ; Meifang Wu ; Zhenghong Zuo ; Zuo ZH(左正宏)
刊名	http://dx.doi.org/10.1371/journal.pone.0050676
	2012
关键词	PERSISTENT ORGANIC POLLUTANTS GENE-EXPRESSION RNA-SEQ TRIBUTYLTIN EXPOSURE SPERMATOGENESIS TRIPHENYLTIN DISCOVERY PROTEINS HORMONE
英文摘要	Background: The rockfish Sebastiscus marmoratus, which is widely distributed in the East Sea and the South Sea of China, is a sensitive model for the toxic effects and mechanisms of marine contaminants. To gain a global view of the molecular mechanism(s) whereby gene expression may influence sexual dimorphism in S. marmoratus, and to develop a database for further toxicological studies, we performed a large-scale transcriptome study. Methodology/Principal Findings: The Illumina DNA sequencing platform was employed to obtain 27,559,578 and 25,821,126 reads from two cDNA libraries generated from adult male and female S. marmoratus, respectively. Transcriptome de novo assembly was carried out with the short reads assembling program-SOAPdenovo. The reads assembled into 78,675 unigenes, of which 38,677 showed homology to existing protein sequences. Clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed to better understand the functions of these unigenes. There were 1,209 potential sex differentially expressed unigenes, with 1,049 predicted to be differentially expressed in females and 160 in males. Fifteen randomly chosen unigenes were confirmed using real-time PCR as female or male predominantly expressed genes. Conclusions/Significance: This is the first report of an annotated transcriptome of S. marmoratus and identification of sex differentially expressed genes. These data will be of interest to researchers using this model. This work also provides an archive for future studies in molecular mechanisms of sexual dimorphism and evolution and can be used in comparative studies of other fish.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65084]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Lingbin Sun,Chonggang Wang,Lixing Huang,et al. Transcriptome Analysis of Male and Female Sebastiscus marmoratus[J]. http://dx.doi.org/10.1371/journal.pone.0050676,2012.
APA	Lingbin Sun,Chonggang Wang,Lixing Huang,Meifang Wu,Zhenghong Zuo,&左正宏.(2012).Transcriptome Analysis of Male and Female Sebastiscus marmoratus.http://dx.doi.org/10.1371/journal.pone.0050676.
MLA	Lingbin Sun,et al."Transcriptome Analysis of Male and Female Sebastiscus marmoratus".http://dx.doi.org/10.1371/journal.pone.0050676 (2012).