Microsatellite alterations in phenotypically normal esophageal squamous epithelium and metaplasia-dysplasia-adenocarcinoma sequence

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	Microsatellite alterations in phenotypically normal esophageal squamous epithelium and metaplasia-dysplasia-adenocarcinoma sequence
	Cai, J. C. ; Liu, D. ; Liu, K. H. ; Zhang, H. P. ; Zhong, S. ; Xia, N. S. ; Xia NS(夏宁邵)
刊名	http://dx.doi.org/10.3748/wjg.14.4070
	2008-07-07
关键词	MICRODISSECTED ENDOSCOPIC BIOPSIES BARRETT-ESOPHAGUS CELL CARCINOMA GENETIC PROGRESSION INSTABILITY CANCER P53 DEFICIENCY EXPRESSION EVOLUTION
英文摘要	AIM: To investigate the microsatellite alterations in phenotypically normal esophageal squamous epithelium and meta plasia-dysplasia-adenocarcinoma sequence. METHODS: Forty-one specimens were obtained from esophageal cancer (EC) patients. Histopathological assessment identified 23 squamous cell carcinomas (SCC) and 18 adenocarcinomas (ADC), including only 8 ADC with Barrett esophageal columnar epithelium (metaplasia) and dysplasia adjacent to ADC. Paraffin-embedded normal squamous epithelium, Barrett esophageal columnar epithelium (metaplasia), dysplasia and esophageal tumor tissues were dissected from the surrounding tissues under microscopic guidance. DNA was extracted using proteinase K digestion buffer, and DNA was diluted at 1:100, 1:1000, 1:5000, 1:10000 and 1:50000, respectively. Seven microsatellite markers (D2S123, D3S1616, D3S1300, D5S346, D17S787, D18S58 and BATRII loci) were used in this study. Un-dilution and dilution polymerase chain reactions (PCR) were performed, and microsatellite analysis was carried out. RESULTS: No statistically significant difference was found in microsatellite instability (MSI) and loss of heterozygosity (LOH) of un-diluted DNA between SCC and ADC. The levels of MSI and LOH were high in the meta plasia-dysplasia-adenocarcinoma sequence of diluted DNA. The more the diluted DNA was, the higher the rates of MSI and LOH were at the above 7 loci, especially at D3S1616, D5S346, D2S123, D3S1300 and D18S58 loci. CONCLUSION: The sequence of metaplasia-dysplasia-adenocarcinoma is associated with microsatellite alterations, including MSI and LOH. The MSI and LOH may be the early genetic events during esophageal carcinogenesis, and genetic alterations at the D3S1616, D5S346 and D3S123 loci may play a role in the progress of microsatellite alterations. (C) 2008 The WJG Press. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65042]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Cai, J. C.,Liu, D.,Liu, K. H.,et al. Microsatellite alterations in phenotypically normal esophageal squamous epithelium and metaplasia-dysplasia-adenocarcinoma sequence[J]. http://dx.doi.org/10.3748/wjg.14.4070,2008.
APA	Cai, J. C..,Liu, D..,Liu, K. H..,Zhang, H. P..,Zhong, S..,...&夏宁邵.(2008).Microsatellite alterations in phenotypically normal esophageal squamous epithelium and metaplasia-dysplasia-adenocarcinoma sequence.http://dx.doi.org/10.3748/wjg.14.4070.
MLA	Cai, J. C.,et al."Microsatellite alterations in phenotypically normal esophageal squamous epithelium and metaplasia-dysplasia-adenocarcinoma sequence".http://dx.doi.org/10.3748/wjg.14.4070 (2008).