QQ客服 官方微博 反馈留言

	Genes involved in alkane degradation in the Alcanivorax hongdengensis strain A-11-3
	Wang, Wanpeng ; Wang WP(王万鹏) ; Shao, Zongze ; Shao ZZ(邵宗泽)
	2012-04
关键词	Marine oil pollution Biodegradation Alkane hydroxylase Gene cluster Alcanivorax hongdengensis
英文摘要	Alcanivorax hongdengensis A-11-3 is a newly identified type strain isolated from the surface water of the Malacca and Singapore Straits that can degrade a wide range of alkanes. To understand the degradation mechanism of this strain, the genes encoding alkane hydroxylases were obtained by PCR screening and shotgun sequencing of a genomic fosmid library. Six genes involved in alkane degradation were found, including alkB1, alkB2, p450-1, p450-2, p450-3 and almA. Heterogeneous expression analysis confirmed their functions as alkane oxidases in Pseudomonas putida GPo12 (pGEc47 Delta B) or Pseudomonas fluorescens KOB2 Delta 1. Q-PCR revealed that the transcription of alkB1 and alkB2 was enhanced in the presence of n-alkanes C-12 to C-24; three p450 genes were up-regulated by C-8-C-16 n-alkanes at different levels, whereas enhanced expression of almA was observed when strain A-11-3 grew with long-chain alkanes (C-24 to C-36). In the case of branched alkanes, pristane significantly enhanced the expression of alkB1, p450-3 and almA. The six genes enable strain A-11-3 to degrade short (C-8) to long (C-36) alkanes that are straight or branched. The ability of A. hongdengensis A-11-3 to thrive in oil-polluted marine environments may be due to this strain's multiple systems for alkane degradation and its range of substrates.; National Science Foundation of China [41176154, 41106151]; International Sci & Tech cooperation Program of China [2010DFB93670]; Sci & Tech Program of Fujian Province of China [2009H0029]
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1007/s00253-011-3818-x]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Wang, Wanpeng,Wang WP,Shao, Zongze,et al. Genes involved in alkane degradation in the Alcanivorax hongdengensis strain A-11-3[J],2012.
APA	Wang, Wanpeng,王万鹏,Shao, Zongze,&邵宗泽.(2012).Genes involved in alkane degradation in the Alcanivorax hongdengensis strain A-11-3..
MLA	Wang, Wanpeng,et al."Genes involved in alkane degradation in the Alcanivorax hongdengensis strain A-11-3".(2012).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。