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	LTB的表达及其粘膜免疫佐剂活性分析; Expression and Analysis of Mucosal Immunoadjuvant Activity of LTB
	张国广 ; 罗茂春(龙岩学院生命科学学院) ; 董艳美 ; 陈亮
	2012-09
关键词	LTB表达 GM1-ELISA 粘膜免疫佐剂活性 LTB expression GM1-ELISA mucosal immunoadjuvant activity
英文摘要	通信作者:chenlg@xmu.edu.cn; [中文文摘]粘膜免疫是预防一些通过吸附粘膜组织侵入机体病原的很好措施,但粘膜免疫通常需要粘膜免疫佐剂,大肠杆菌(Escherichia coli)不耐热肠毒素的B亚基具有很好的粘膜免疫佐剂活性.本研究从大肠杆菌195菌株中扩增出LT(heat-labile enterotoxin)基因,测序后将其B亚基基因与pET32a连接构建了重组质粒pET32a-LTB,SDS-PAGE显示LTB(heat-labile enterotoxin B subunit)在原核细胞中得到表达,Western blotting和人神经节苷脂结合酶联免疫吸附试验(GM1-ELISA)结果表明,重组LTB具有抗原性和GM1结合活性.将其与禽流感M2e表位融合蛋白M2eHBc+混合通过滴鼻途径免疫小鼠,抗体检测结果表明,所表达的LTB能很好地提高共免疫抗原的粘膜和系统免疫应答.[英文文摘]Mucosal immunity is a good measure for preventing pathogenic agents that occur at or emanate from mucosal surfaces invaded the body,but mucosal immunity usually need to mucosal immunoadjuvant.Escherichia coli heat-labile enterotoxin B subunit(LTB) has a good mucosal immunoadjuvant activity.LT gene was amplified by polymerase chain reaction from an enterotoxigenic E.coli 195 strain in this study.Prokaryotic expression vector pET32a-LTB was constructed by LTB subunit gene and pET32a connection after LT gene was sequenced.SDS-PAGE displayed that LTB subunit are expressed in E.coli cells and recombinant LTB inherited the antigenicity and GM1-binding activity of cholerae enterotoxin by Western blotting and GM1-ELISA assay.The mice immunization with mixing of recombinant LTB and avian infuenza virus M2eepitope fusion protein M2eHBc+by intranasal dropping way revealed that LTB can significantly enhance the mucosal and systemic immune responses of mice to co-administered antigen.; 福建省科技厅重点项目(2009N0051); 福建省自然科学基金项目(2010J01240);; 厦门市科技计划项目(3502Z20103007)
语种	中文
出版者	《厦门大学学报(自然科学版)》编辑部
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/15600]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	张国广,罗茂春(龙岩学院生命科学学院),董艳美,等. LTB的表达及其粘膜免疫佐剂活性分析, Expression and Analysis of Mucosal Immunoadjuvant Activity of LTB[J],2012.
APA	张国广,罗茂春,董艳美,&陈亮.(2012).LTB的表达及其粘膜免疫佐剂活性分析..
MLA	张国广,et al."LTB的表达及其粘膜免疫佐剂活性分析".(2012).

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