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	Application of europium(III) chelates-bonded silica nanoparticle in time-resolved immunofluorometric detection assay for human thyroid stimulating hormone
	Zhou, Yulin ; Xia, Xiaohu ; Xu, Ye ; Ke, Wei ; Yang, Wei ; Li, Qingge ; Li QG(李庆阁)
	2012-04-13
关键词	Europium(III) chelate Silica nanoparticle Human thyroid stimulating hormone Time-resolved immunofluorometric assay
英文摘要	Eu(III) chelate-bonded silica nanoparticle was used as a fluorescent label to develop a highly sensitive time-resolved immunofluorometric assay (TrlFA) for human thyroid stimulating hormone (hT:SH). The limit of detection of the assay calculated according to the 2SD method was 0.0007 mlU L-1 and became 0.003 mlU L-1 when serum-based matrix was used for calibrators, indicating that this TrlFA is comparable with the most sensitive assays. The linear range was from 0.005 to 100 mIU L-1 of hTSH with coefficient of variation between 1.9% and 8.3%. The correlation study using 204 blood spot samples from newborns showed that the results from this new method were coincident with that of the commercial dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) system, with a correlation coefficient of 0.938. The fluorescent nanoparticle label allows directly reading the fluorescent signal, omitting the signal development step required for the DELFIA system, and the whole procedure of this assay is fulfilled within 2 h. Thus, we developed a novel, sensitive, quantitative and simple nanoparticle label-based TrlFA assay, suitable for routine application in hTSH screening of neonatal hypothyroidism. (c) 2012 Elsevier B.V. All rights reserved.; Fujian Innovation Project of Medical Sciences [2007-CXB-12]
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/j.aca.2012.01.065]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhou, Yulin,Xia, Xiaohu,Xu, Ye,et al. Application of europium(III) chelates-bonded silica nanoparticle in time-resolved immunofluorometric detection assay for human thyroid stimulating hormone[J],2012.
APA	Zhou, Yulin.,Xia, Xiaohu.,Xu, Ye.,Ke, Wei.,Yang, Wei.,...&李庆阁.(2012).Application of europium(III) chelates-bonded silica nanoparticle in time-resolved immunofluorometric detection assay for human thyroid stimulating hormone..
MLA	Zhou, Yulin,et al."Application of europium(III) chelates-bonded silica nanoparticle in time-resolved immunofluorometric detection assay for human thyroid stimulating hormone".(2012).

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