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	Arachidonic acid regulation of the cytosolic phospholipase A(2 alpha)/cyclooxygenase-2 pathway in mouse endometrial stromal cells
	Zhao, Zhen-Ao ; Zhang, Zhi-Rong ; Xu, Xiu ; Deng, Wen-Bo ; Li, Ming ; Leng, Jing-Yu ; Liang, Xiao-Huan ; Yang, Zeng-Ming ; Yang ZM(杨增明)
	2012-05
关键词	Uterus embryo implantation decidualization arachidonic acid
英文摘要	Objective: To investigate the role of arachidonic acid (AA) in mouse endometrial stromal cells. Design: Experimental animal study. Setting: University research laboratory. Animal(s): Sexually mature female CD1-strain mice. Intervention(s): Primary culture of endometrial stromal cells. Main Outcome Measure(s): Western blot and real-time polymerase chain reaction for gene expression and/or phophorylation analysis. Luciferase assay for Cox-2 promoter analysis. Result(s): AA-derived prostaglandins play important roles during embryo implantation and decidualization. However, the function of AA itself in reproduction is largely unknown. In this study, exogenous AA stimulated cPLA(2 alpha) phosphorylation and COX-2 expression, mainly through ERK1/2 in mouse endometrial stromal cells, and p38 inhibitor modestly inhibited cPLA(2 alpha) phosphorylation induced by AA. The induction of COX-2 by AA was diminished by short interfering RNA against C/EBP beta and inhibitory C/EBP beta (LIP). C/EBPb binding site at -872- -864 of Cox-2 promoter contributes to Cox-2 promoter activation induced by C/EBPb transfection. The expression of C/EBPb protein induced by AA was inhibited by p38 inhibitor, and the phosphorylation of C/EBP beta induced by AA was inhibited by p38 inhibitor and ERK1/2 inhibitor. A nonmetabolized analogue of AA (ETYA) also enhanced cPLA(2 alpha) phosphorylation and COX-2 expression. The activation of cPLA(2 alpha)/COX-2 by AA was not inhibited by COX inhibitor indomethacin. Conclusion(s): AA can induce cPLA(2 alpha)/COX-2 pathway activation in mouse endometrial stromal cells. (Fertil Steril (R) 2012;97:1199-1205. (C) 2012 by American Society for Reproductive Medicine.); National Basic Research Program of China [2011CB944402]; National Natural Science Foundation of China [30930013, 31071276]
语种	英语
出版者	ELSEVIER SCIENCE INC
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/j.fertnstert.2012.02.011]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhao, Zhen-Ao,Zhang, Zhi-Rong,Xu, Xiu,et al. Arachidonic acid regulation of the cytosolic phospholipase A(2 alpha)/cyclooxygenase-2 pathway in mouse endometrial stromal cells[J],2012.
APA	Zhao, Zhen-Ao.,Zhang, Zhi-Rong.,Xu, Xiu.,Deng, Wen-Bo.,Li, Ming.,...&杨增明.(2012).Arachidonic acid regulation of the cytosolic phospholipase A(2 alpha)/cyclooxygenase-2 pathway in mouse endometrial stromal cells..
MLA	Zhao, Zhen-Ao,et al."Arachidonic acid regulation of the cytosolic phospholipase A(2 alpha)/cyclooxygenase-2 pathway in mouse endometrial stromal cells".(2012).

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