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	Cathepsin B-like and hemoglobin-type cysteine proteases: Stage-specific gene expression in Angiostrongy cantonensis
	Ni, Fang ; Wang, Yinan ; Zhang, Jing ; Yu, Liang ; Fang, Wenzhen ; Fang WZ(方文珍) ; Luo, Damin ; Luo DM(罗大民)
	2012-08
关键词	Stage-specific Cathepsin B-like cysteine protease Hemoglobin-type cysteine protease Angiostrongylus cantonensis Reverse transcription-PCR Real-time PCR
英文摘要	Three cysteine protease genes, cathepsin B-like enzyme gene 1, 2 (AC-cathB-1, AC-cathB-2) and hemoglobin-type cysteine protease gene (AC-hem) were isolated and described from Angiostrongylus cantonensis adult. The deduced amino acid sequence of Ac-cathB-1 and AC-cathB-2 contain all of the conserved regions of cathepsin B. AC-cathB-2 is similar to a host intrusion-related cysteine protease B from Parelaphostrongylus tenuis, and the AC-hem shares high similarity to legumain from Haemonchus contortus. AC-cathB-1 was expressed significantly higher in L1 as compared with AC-hem, the AC-cathB-2 followed; AC-cathB-2 transcripts in L3 were found increased rapidly and obviously abundant, suggesting that AC-cathB-1 and AC-cathB-2 may play an important role in intermediate and final host invasion, separately. The cysteine protease genes were more or less expressed in adult stage excepted for AC-cathB-2. As the AC-cathB-1 and AC-hem highly expressed in adult worms, suggesting AC-hem may activate AC-cathB-1 which involved in the host invasion and feeding process. (C) 2012 Elsevier Inc. All rights reserved.; Natural Science Foundation of China 81171595 Natural Science Foundation of Fujian Province 2010J01229
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/j.exppara.2012.05.014]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ni, Fang,Wang, Yinan,Zhang, Jing,et al. Cathepsin B-like and hemoglobin-type cysteine proteases: Stage-specific gene expression in Angiostrongy cantonensis[J],2012.
APA	Ni, Fang.,Wang, Yinan.,Zhang, Jing.,Yu, Liang.,Fang, Wenzhen.,...&罗大民.(2012).Cathepsin B-like and hemoglobin-type cysteine proteases: Stage-specific gene expression in Angiostrongy cantonensis..
MLA	Ni, Fang,et al."Cathepsin B-like and hemoglobin-type cysteine proteases: Stage-specific gene expression in Angiostrongy cantonensis".(2012).

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