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	秀丽线虫中p53/cep-1基因克隆、表达及其对寿命的影响; Cloning,Expressing of Caenorhabditis elegans p53/cep-1 and the Effect on Lifespan
	苏小珊 ; 杨玉荣
	2010-03
关键词	Caenorhabditis elegans p53/cep-1 克隆 表达 RNAi 寿命
英文摘要	通i讯作者. Tel: 0592-2181792, E-mail:yryang@xmu.edu.cn; [中文摘要]Caenorhabditis elegans中的cep-l基因与哺乳动物中的肿瘤抑制因子p53同源,能够引发由DNA损伤导致的细胞凋亡。cep-l基因与寿命的关系研究并不多。我们利用C.elegans的cDNA进行PCR扩增得到cep-l的片段并构建到表达载体以及RNA干扰载体上,经过IPTG诱导表达得到了约90kDa的CEP-1融合蛋白。同时对C.elegans三种虫株:野生型,daf-2(e1370),daf-16(e1038)进行RNAi(RNA-mediated interference),发现cep-l RNAi之后野生型的寿命显著延长,另外两种虫株的寿命没有显著改变,并以RT-PCR的方法检测了cep-l在这三种虫株中的表达情况。[英文摘要] Caenorhabditis elegans cep-l was similar to its mammalian counterpart, tumor suppressor p53.cep-l was amplified from C.elegans cDNA by PCR and cloned into expression vector and RNA interference vector.The full length of cep-l cDNA was 2106bp, containing a 5'untranslated region of 27 bp and a 3'untranslated region of 144 bp. The open reading frame was 1935bp which predicted encoding a 645 amino acids protein. CEP-1 recombinant protein was obtained by IPTG induced in Escherichia coli. At the same time, we investigated the effect of p53lcep-l on C.elegans lifespan in wild type, daf-2 (e1370), daf-16 (e1038) strains by RNAi (RNA-mediated interference), and found that the lifespan of wild type was prolonged significantly in cep-l RNAi worm, in contrast, the lifespan of the other strains have no difference with the RNAi control worms. And the transcription level of cep- I in N2, daf-2 (e1370) and daf-16 (e1038) was determined by RT-PCR.; 国家自然科学基金(No.30972181);厦门大学新世纪优秀人才支持计划资助项目
语种	中文
出版者	《中国细胞生物学学报》编辑部
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/14598]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	苏小珊,杨玉荣. 秀丽线虫中p53/cep-1基因克隆、表达及其对寿命的影响, Cloning,Expressing of Caenorhabditis elegans p53/cep-1 and the Effect on Lifespan[J],2010.
APA	苏小珊,&杨玉荣.(2010).秀丽线虫中p53/cep-1基因克隆、表达及其对寿命的影响..
MLA	苏小珊,et al."秀丽线虫中p53/cep-1基因克隆、表达及其对寿命的影响".(2010).

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