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	产氢细菌Enterbacter sakazakii HP的分离及产氢特性; Isolation and Characterization of a H2 -producing Strain Enterbacter sakazakii HP
	常娥 ; 齐亚林 ; 邬小兵 ; 徐惠娟 ; 龙敏南
	2007-04
关键词	Enterbacter sakazakii 分离鉴定 放氢特性 Enterbacter sakazakii HP Isolation and identification Hydrogen production
英文摘要	通讯作者􀀁 Tel 0592-2185731, E-mail： longmn@ xmu.edu.cn; [中文文摘]在自然环境中分离到一株具有高产氢活性的微生物菌株,经细菌鉴定仪及16S rRNA基因序列分析,鉴定该菌株为Enterbacter sakazakii HP。分析了起始pH值、反应温度、碳源、起始糖浓度、起始氧浓度及菌体密度等因素对菌株产氢活性的影响。研究表明,该菌株发酵产氢较适合的条件为:以葡萄糖为产氢底物,起始pH值8.0,菌体密度OD600=0.7,反应温度35℃,糖浓度为0.1mol/L,氧浓度为0%的条件下,此时产氢菌株的最高产氢活性为5.34μmolH2/h.mgdw,氢的得率为1.94molH2/mol葡萄糖。[英文文摘]A H2 -producing bacterial strain was newly isolated and identified as Enterbacte sakazakii HP by 16S rDNA sequence analysis and detection by BBLCRYSTAL AUTOREADER.Various factors,including substrates and its concentration,initial pH,temperature and oxygen,on the hydrogen production of E.sakazakii HP have been studied extensively.Among several sugars,glucose was the favorite substrate for hydrogen production.The optimum condition for hydrogen production by Enterbacte sakazakii HP was achieved as:initial pH8.0,cell density OD600 =0.7,temperature 35℃,glucose concentration 0.1 mol/L,oxygen concentration 0%.Under batch fermentative hydrogen production conditions,the maximal hydrogen production activity and hydrogen yield were obtained as 5.34μmol H2 /h·mg dw and 1.94mol H2 /mol glucose,respectively.The research results suggest that Enterbacter sakazakii HP is an ideal candidater for biological hydrogen production.; 国家自然科学基金项目(No30470395);厦门市科技项目(No3502Z20041070);厦门大学新世纪优秀人才计划项目;福建省青年科技人才创新项目(No2005J003)
语种	中文
出版者	《微生物学通报》编辑部
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/13849]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	常娥,齐亚林,邬小兵,等. 产氢细菌Enterbacter sakazakii HP的分离及产氢特性, Isolation and Characterization of a H2 -producing Strain Enterbacter sakazakii HP[J],2007.
APA	常娥,齐亚林,邬小兵,徐惠娟,&龙敏南.(2007).产氢细菌Enterbacter sakazakii HP的分离及产氢特性..
MLA	常娥,et al."产氢细菌Enterbacter sakazakii HP的分离及产氢特性".(2007).

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