QQ客服 官方微博 反馈留言

	Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum
	Yang, Yurong ; Yang YR(杨玉荣) ; Qin, Weiwen ; Wei, Hua ; Ying, Jianxi ; Zhen, Jing
	2011-09-07
关键词	Ancylostoma caninum Cathepsin B proteinase Expressing Localization Eggs Larval stage
英文摘要	Cathepsin B proteinase constitutes a large multigenes family in parasitic and non-parasitic nematodes. The localization of cathepsin B proteinases (AcCP-1 and AcCP-2) in adult worm of Ancylostoma caninum has been characterized (Harrop et al., 1995), but the localization and function in eggs and larval stages remained undiscovered. Here we described the expressing of cathepsin B proteinase (AcCP-2) in Escherichia colt, and immuno-localization of cathepsin B proteinase in eggs and larvae stages of A. caninum. A cDNA fragment encoding a cathepsin B proteinase (AcCP-2) was cloned from A. caninum and expressed in E. colt. Gelatin digestion showed that recombinant cathepsin B proteinase (AcCP-2) has protease activity. The protein level of cathepsin B proteinase in larval and adult worm was detected by western blot. The immuno-localization of cathepsin B proteinase in eggs and larval stages was characterized. The expression of cathepsin B proteinase was more abundant in eggs and larvae stages of A. caninum. It implied that cathepsin B proteinase might play roles in the early development of A. caninum. (C) 2011 Elsevier Inc. All rights reserved.; China National Nature Science foundation[30972181]; Natural Science Foundation of Fujian Province[C0320001]; Xiamen Science Technology Program[3502Z20061098]; Science and Technology Foundation[06C26223511306]
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.exppara.2011.08.018]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Yang, Yurong,Yang YR,Qin, Weiwen,et al. Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum[J],2011.
APA	Yang, Yurong,杨玉荣,Qin, Weiwen,Wei, Hua,Ying, Jianxi,&Zhen, Jing.(2011).Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum..
MLA	Yang, Yurong,et al."Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum".(2011).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。