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	Modification and Modificatory Kinetics of the Active Center of Prawn beta-N-Acetyl-D-glucosaminidase
	Xie, Xiao-Lan ; Huang, Qian-Sheng ; Wang, Ye ; Ke, Cai-Huan ; Chen, Qing-Xi ; Chen QX(陈清西)
	2009
关键词	beta-N-Acetyl-D-glucosaminidase Litopenaeus vannamei Essential groups Modification and Modificatory kinetics
英文摘要	beta-N-acetyl-D-glucosaminidase (NAGase, EC3.2.1.52) plays important role in molting, digestion of chitinous foods, and defense systems against parasites in prawn (Litopenaeus vannamei). However, study on functional groups and catalytic mechanism of NAGase are yet limited. The modification of the active center of NAGase from prawn has been first studied. The results demonstrate that the disulfide bonds and the carbamidine groups of arginine residues are not essential to the enzyme's activity. The modification of indole group of tryptophan of the enzyme by N-bromosuccinimide (NBS) can lead to the complete inactivation, accompanying the absorption decreasing at 276 run, indicating that tryptophan is essential residue to the enzyme. The modificatory kinetics of NAGase in the appropriate concentrations of NBS solution has been studied and the numbers of essential tryptophan residues have been determined using the kinetic method of the substrate reaction. The result shows that only one tryptophan residue is essential for enzyme activity. And the modifications of histidine, lysine residue, and the carboxyl groups also inactivate the enzyme completely or incompletely. The results showed that the carboxyl groups of acidic amino acid, imidazole groups of histidine residue, amino groups of lysine residue, and indole group of tryptophan were essential for the activity of enzyme.; China Postdoctoral Science Foundation [20070410806]; Science and Technology Foundation of Fujian Province [2006N0067]; Program of the Key Laboratory of Fujian Province for Molecular Biology and Medical Chemistry
语种	英语
出版者	ADENINE PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/11306]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Xie, Xiao-Lan,Huang, Qian-Sheng,Wang, Ye,et al. Modification and Modificatory Kinetics of the Active Center of Prawn beta-N-Acetyl-D-glucosaminidase[J],2009.
APA	Xie, Xiao-Lan,Huang, Qian-Sheng,Wang, Ye,Ke, Cai-Huan,Chen, Qing-Xi,&陈清西.(2009).Modification and Modificatory Kinetics of the Active Center of Prawn beta-N-Acetyl-D-glucosaminidase..
MLA	Xie, Xiao-Lan,et al."Modification and Modificatory Kinetics of the Active Center of Prawn beta-N-Acetyl-D-glucosaminidase".(2009).

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