CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE-RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS1 | |
Xie, CT(The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College,) ; Chen, CS(The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College,) ; Xu, Y ; Xu Y(徐燕) ; Ji, DH(The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College,) | |
2010 | |
关键词 | DH population genetic linkage map Porphyra haitanensis SRAP SSR |
英文摘要 | Molecular markers and molecular genetic maps are prerequisites for molecular breeding in any plant species. A comprehensive genetic linkage map for cultivated Porphyra haitanensis T. J. Chang et B. F. Zheng has not yet been developed. In this study, 157 double haploid (DH) lines [derived from a YSIII (wildtype) x RTPM (red-type artificial pigmentation mutant) cross] were used as a mapping population in P. haitanensis. A total of 60 pairs of sequence-related amplified polymorphism (SRAP) primers and 39 pairs of simple sequence repeat (SSR) primers were used to detect polymorphisms between the two parents. Fifteen SRAP and 16 SSR polymorphic primer pairs were selected to analyze the DH population. A linkage genetic map comprising 67 SRAP markers and 20 SSR markers in five linkage groups, with a total length of 830.6 cM and an average of 10.13 cM between markers, was constructed. The markers were distributed evenly in all linkage groups without clustering. The linkage groups comprised 12-23 markers ranging in length from 134.2 to 197.3 cM. The estimated genome length of P. haitanensis was 942.4 cM, with 88.1% coverage. This is the first report of a comprehensive genetic map in P. haitanensis. The map presented here will provide a basis for the development of high-density genetic linkage maps and lay the foundation for molecular breeding work in P. haitanensis.; National High Technology Research & Development Program of China ("863" Program) [2006AA10A413]; National Natural Science Foundation of China [40676077, 40806065]; National Natural Science Foundation of Fujian Province, China [2007J0064]; Science and Technological Platform of Fujian Province, China [2007N2011]; Foundation for Young Professors of Jimei University, China [2007C002]; Foundation of Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fujian Province University [2008J202] |
语种 | 英语 |
出版者 | WILEY-BLACKWELL PUBLISHING, INC |
内容类型 | 期刊论文 |
源URL | [http://dx.doi.org/doi:10.1111/j.1529-8817.2010.00855.x] ![]() |
专题 | 生命科学-已发表论文 |
推荐引用方式 GB/T 7714 | Xie, CT,Chen, CS,Xu, Y,et al. CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE-RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS1[J],2010. |
APA | Xie, CT,Chen, CS,Xu, Y,徐燕,&Ji, DH.(2010).CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE-RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS1.. |
MLA | Xie, CT,et al."CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE-RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS1".(2010). |
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