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	EPR study of soluble hydrogenase from photosynthetic bacteria Chromatium vinosum
	Long, MN ; Long MN(龙敏南) ; Su, WJ ; Su WJ(苏文金) ; Albracht, SPJ（E.C.SlaterInstitute University of Amsterdam, Amsterdam, the Netherlands） ; Zhang, FZ ; Zhang FZ(张凤章) ; Xu, LS ; Xu LS(许良树)
	2001
关键词	Chromatium vinosum soluble hydrogenase EPR spectra
英文摘要	A soluble hydrogenase(SH) was purified from Chromatium vinosum by five step chromatography(DE-23, TSK-DEAE(I), Ultragel AcA-44, TSK-DEAE(I), Superdex TM75) with a specific activity of 8.4 mu mol H-Z/(min.mg prot). The oxidized SH yield two Ni( I) EPR(electron paramagnetic resonance) signals (g(x,y,z)=2.37, 2.16, 2.016 and g(x,y,z)=2.30, 2.23, 2.016) at 45 K which occurred in the other NiFe-hydrogenases. However, no [3Fe-4S] cluster EPR signal was obtained at 10 K. When the SH was reduced by H-2(over night at 8 degreesC), the Ni(I) EPR signals disappeared, and an EPR signal from a reduced[4Fe-4S] cluster appeared (g(x,y,z)=1.88, 1.90, 2.045). The results show that the soluble hydrogenase from C. vinosum is a new NiFe-hydrogenase which catalyzes H-2-production.
语种	英语
出版者	HIGHER EDUCATION PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/11082]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Long, MN,Long MN,Su, WJ,et al. EPR study of soluble hydrogenase from photosynthetic bacteria Chromatium vinosum[J],2001.
APA	Long, MN.,龙敏南.,Su, WJ.,苏文金.,Albracht, SPJ.,...&许良树.(2001).EPR study of soluble hydrogenase from photosynthetic bacteria Chromatium vinosum..
MLA	Long, MN,et al."EPR study of soluble hydrogenase from photosynthetic bacteria Chromatium vinosum".(2001).

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