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	Induction of apoptosis by TPA and VP-16 is through translocation of TR3
	Liu, S ; Liu S(刘苏) ; Wu, Q ; Wu Q(吴乔) ; Ye, XF ; Cai, JH ; Huang, ZW ; Su, WJ ; Su WJ(苏文金)
	2002
英文摘要	AIM: To investigate the role of TR3 in induction of apoptosis in gastric cancer cells. METHODS: Human gastric cancer cell line, MGC80-3, was used., Expression of TR3 mRNA and its protein was detected by Northern blot and Western blot. Localization of TR3 protein was showed by immunofluorescence analysis under laser-scanning confocal microscope. Apoptotic morphology was observed by DAPI fluorescence staining, and apoptotic index was counted among 1000 cells randomly. Stable transfection assay was carried out by Lipofectamine. RESULTS: Treatment of MGC80-3 cells with TPA and VP-16 resulted in apoptosis, accompanied by the repression of Bcl-2 protein in a time-dependent manner. At the same time, TPA and VP-16 also up-regulated expression level of TR3 mRNA in MGC80-3 cells that expressed TR3 mRNA. When antisense-TR3 expression vector was transfected into the cells, expression of TR3 protein was repressed. In this case, TPA and VP-16 did not induce apoptosis. In addition, TPA and VP-16-induced apoptosis involved in translocation of TR3. In MGC80-3 cells, TR3 localized concentrative in nucleus, after treatment of cells with TPA and VP-16, TR3 translocated from nucleus to cytosol obviously. However, when this nuclear translocation was blocked by LMB, apoptosis,was not occurred in MGC80-3 cells even in the presence of TPA and VP-16. CONCLUSION: Induction of apoptosis by TPA and VP-16 is through induction of TR3 expression and translocation of TR3 from nucleus to cytosol, which may be a novel signal pathway for TR3, and represent the new biological function of TR3 to exert its effect on apoptosis in gastric cancer cells.
语种	英语
出版者	W J G PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/11061]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liu, S,Liu S,Wu, Q,et al. Induction of apoptosis by TPA and VP-16 is through translocation of TR3[J],2002.
APA	Liu, S.,刘苏.,Wu, Q.,吴乔.,Ye, XF.,...&苏文金.(2002).Induction of apoptosis by TPA and VP-16 is through translocation of TR3..
MLA	Liu, S,et al."Induction of apoptosis by TPA and VP-16 is through translocation of TR3".(2002).

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