QQ客服 官方微博 反馈留言

	Distinct role and functional mode of TR3 and RAR alpha in mediating ATRA-induced signalling pathway in breast and gastric cancer cells
	Ye, XF ; Wu, Q ; Wu Q(吴乔) ; Liu, S ; Lin, XF ; Zhang, B ; Wu, JF ; Cai, JH ; Zhang, MQ ; Su, WJ ; Su WJ(苏文金)
	2004
关键词	TR3 retinoic acid receptor alpha (RAR alpha) retinoid X receptor alpha (RYR alpha) translocation all-trans retinoic acid (ATRA) cancer cell
英文摘要	All-trans retinoic acid (ATRA) affects cell proliferation, differentiation and apoptosis through its receptors, RARs and RXRs. Besides these, other receptors such as orphan receptor TR3, are also involved in the regulatory process of ATRA. However, how different receptors function in response to ATRA is still largely unknown. In the present study, we found that formation of TR3/RXRalpha heterodimers in the nucleus and their subsequent translocation into the cytoplasm, in association with regulation of apoptosis-related proteins Bcl-2, Bcl-xl and Bax, was critical for apoptosis induction by ATRA in breast cancer cells MCF-7. When such translocation was blocked by Leptomycin B (LMB), ATRA-induced apoptosis was consequently abolished. However, in ATRA-induced gastric cancer cells MGC80-3, RXRalpha heterodimerised with RARalpha but not with TR3, and remained in the nucleus exerting its effect on cell cycle regulation. When transfected with antisense-RARalpha, MGC80-3 cells changed from ATRA-sensitive to ATRA-resistant and most cells were arrested in the S phase, implying the importance of RARalpha in cell cycle regulation. Furthermore, we demonstrated that the effects of ATRA depend on the relative levels of TR3, RARalpha and RXRalpha expression in cancer cells. In ATRA-induced MCF-7 cells, highly expressed TR3 favours the formation of TR3/RXRalpha and promotes the TR3/RXRalpha signalling pathway causing apoptosis; while in ATRA-induced MGC80-3 cells, high expression of RARalpha favours the formation of RARalpha/RXRalpha and promotes the RXRalpha/RARalpha signalling pathway in mediating cell cycle regulation. In conclusion, these results reveal the novel mechanism that cellular expression and location of protein is associated with diverse signalling transduction pathways and the resultant physiological process. (C) 2003 Elsevier Science Ltd. All rights reserved.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/S1357-2725(03)00143-2]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ye, XF,Wu, Q,Wu Q,et al. Distinct role and functional mode of TR3 and RAR alpha in mediating ATRA-induced signalling pathway in breast and gastric cancer cells[J],2004.
APA	Ye, XF.,Wu, Q.,吴乔.,Liu, S.,Lin, XF.,...&苏文金.(2004).Distinct role and functional mode of TR3 and RAR alpha in mediating ATRA-induced signalling pathway in breast and gastric cancer cells..
MLA	Ye, XF,et al."Distinct role and functional mode of TR3 and RAR alpha in mediating ATRA-induced signalling pathway in breast and gastric cancer cells".(2004).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。