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	Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation
	Rui, YN（Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong） ; Xu, Z（Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong） ; Lin, SY ; Lin SY(林舒勇) ; Li, QX ; Li QX(李勤喜) ; Rui, HL（Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong） ; Luo, W ; Zhou, HM（Department of Biological Sciences, Tsinghua University） ; Cheung, PY（Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong） ; Wu, ZG（Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong） ; Ye, ZY ; Ye ZY(叶志云) ; Li, P（Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong） ; Han, JH ; Han JH(韩家淮) ; Lin, SC ; Lin SC(林圣彩)
	2004
关键词	apoptosis Axin HIPK2 p53 phosphorylation
英文摘要	Axin and p53 are tumor suppressors, controlling cell growth, apoptosis, and development. We show that Axin interacts with homeodomain-interacting protein kinase-2 (HIPK2), which is linked to UV-induced p53-dependent apoptosis by interacting with, and phosphorylating Ser 46 of, p53. In addition to association with p53 via HIPK2, Axin contains a separate domain that directly interacts with p53 at their physiological concentrations. Axin stimulates p53-dependent reporter transcription in 293 cells, but not in 293T, H1299, or SaOS-2 cells that are defective in p53 signaling. Axin, but not AxinDeltaHIPK2, activates HIPK2-mediated p53 phosphorylation at Ser 46, facilitating p53-dependent transcriptional activity and apoptosis. Specific knockdown of Axin by siRNA reduced UV-induced Ser-46 phosphorylation and apoptosis. Kinase-dead HIPK2 reduced Axin-induced p53-dependent transcriptional activity, indicating that Axin stimulates p53 function through HIPK2 kinase activity. Interestingly, HIPK2DeltaAxin that lacks its Axin-binding region acts as a dominant-positive form in p53 activation, suggesting that the Axin-binding region of HIPK2 is a putative auto-inhibitory domain. These results show that Axin acts as a tumor suppressor by facilitating p53 function through integration of multiple factors.
语种	英语
出版者	NATURE PUBLISHING GROUP
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1038/sj.emboj.7600475]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Rui, YN,Xu, Z,Lin, SY,et al. Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation[J],2004.
APA	Rui, YN.,Xu, Z.,Lin, SY.,林舒勇.,Li, QX.,...&林圣彩.(2004).Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation..
MLA	Rui, YN,et al."Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation".(2004).

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