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	Determination of nucleic acids based on shifting the association equilibrium between a heptamethine cyanine dye and poly-lysine
	Zhu, CQ ; Zhu, SJ（Anhui Normal Univ, Coll Chem & Mat Sci） ; Zheng, H ; Chen, JL ; Li, DH ; Li DH(李东辉) ; Lil, SH ; Xu, JG
	2005
关键词	near-IR fluorescence cyanine poly-lysine nucleic acids
英文摘要	A near-infrared (near-IR) fluorescence recovery method for the determination of nucleic acids is presented. This method employs a two-reagent system composed of anionic heptamethines cyanine (HMC) and polycationic poly-lysine. The fluorescence of HMC, with maximum excitation and emission wavelengths at 778 and 804 nm, respectively, was quenched by poly-lysine in proper concentration, but recovered by adding nucleic acids. Under optimal conditions, the recovered fluorescence was proportional to the concentration of nucleic acids. The calibration graphs are linear over the range of 5-300 ng mL(-1) for herring sperm DNA (FS DNA), 2-100 ng mL(-1) for calf thymus DNA (CT DNA) and 5-500 ng mL(-1) for snake ovum RNA (SO RNA). The corresponding detection limits are 1.49 ng mL(-1) for FS DNA, 0.7 ng mL(-1) for CT DNA and 1.61 ng mL(-1) for SO RNA, respectively. Four synthetic and three real nucleic acid samples were determined with satisfactory results.
语种	英语
出版者	SPRINGER WIEN
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1007/s00604-004-0268-5]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhu, CQ,Zhu, SJ,Zheng, H,et al. Determination of nucleic acids based on shifting the association equilibrium between a heptamethine cyanine dye and poly-lysine[J],2005.
APA	Zhu, CQ.,Zhu, SJ.,Zheng, H.,Chen, JL.,Li, DH.,...&Xu, JG.(2005).Determination of nucleic acids based on shifting the association equilibrium between a heptamethine cyanine dye and poly-lysine..
MLA	Zhu, CQ,et al."Determination of nucleic acids based on shifting the association equilibrium between a heptamethine cyanine dye and poly-lysine".(2005).

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