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	Isolation and characterization of a high H-2-producing strain Klebsiella oxytoca HP1 from a hot spring
	Minnan, L ; Long MN(龙敏南) ; Jinli, H ; Xiaobin, W ; Wu XB(邬小兵) ; Huijuan, X ; Xu HJ(徐惠娟) ; Jinzao, C ; Chuannan, L ; Fengzhang, Z ; Liangshu, X
	2005
关键词	hot spring Klebsiella oxytoca HP1 hydrogen production
英文摘要	A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen production, were investigated. Among different sugars, glucose and sucrose were the preferred substrates for hydrogen production. The optimal starting pH of culture was about 7.0. The activity was drastically reduced in a prolonged fermentation due to the accumulation of organic acids. Increasing temperatures (from 25 to 35 degreesC) improved the hydrogen production activity. K. oxytoca HP1 produced hydrogen under different concentrations (1-10%) of oxygen in the gas phase, indicating that it is highly resistant to oxygen inhibition. Under batch ferment conditions, the maximal hydrogen production activity, rate and yield were obtained as 9.6 mmol/g dw h, 87.5 ml/l h and 1.0 mol/mol glucose (conversion 16.7%), respectively. In continuous hydrogen production, the maximum activity, rate and yield were 15.2 mmol/g dw h, 350.0 ml/l h and 3.6 mol/mol sucrose (conversion 32.5%), respectively. These results indicate that K. oxytoca HP I is an ideal hydrogen producer. (C) 2004 Elsevier SAS. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.resmic.2004.08.004]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Minnan, L,Long MN,Jinli, H,et al. Isolation and characterization of a high H-2-producing strain Klebsiella oxytoca HP1 from a hot spring[J],2005.
APA	Minnan, L.,龙敏南.,Jinli, H.,Xiaobin, W.,邬小兵.,...&Liangshu, X.(2005).Isolation and characterization of a high H-2-producing strain Klebsiella oxytoca HP1 from a hot spring..
MLA	Minnan, L,et al."Isolation and characterization of a high H-2-producing strain Klebsiella oxytoca HP1 from a hot spring".(2005).

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