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	Preparation of His-tagged armored RNA phage particles as a control for real-time reverse transcription-PCR detection of severe acute respiratory syndrome coronavirus
	Cheng, Yangjian ; Niu, Jianjun（Xiamen Center for Disease Control and Prevention） ; Zhang, Yongyou ; Huang, Jianwei（Xiamen Center for Disease Control and Prevention） ; Li, Qingge ; Li QG(李庆阁)
	2006
英文摘要	Armored RNA has been increasingly used as both an external and internal positive control in nucleic acid-based assays for RNA virus. In order to facilitate armored RNA purification, a His(6) tag was introduced into the loop region of the MS2 coat protein, which allows the exposure of multiple His tags on the surface during armored RNA assembly. The His-tagged armored RNA particles were purified to homogeneity and verified to be free of DNA contamination in a single run of affinity chromatography. A fragment of severe acute respiratory syndrome coronavirus (SARS-CoV) genome targeted for SARS-CoV detection was chosen for an external positive control preparation. A plant-specific gene sequence was chosen for a universal noncompetitive internal positive control preparation. Both controls were purified by Co2+ affinity chromatography and were included in a real-time reverse transcription-PCR assay for SARS-CoV. The noncompetitive internal positive control can be added to clinical samples before RNA extraction and enables the identification of potential inhibitive effects without interfering with target amplification. The external control could be used for the quantification of viral loads in clinical samples.
语种	英语
出版者	AMER SOC MICROBIOLOGY
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1128/JCM.00713-06]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Cheng, Yangjian,Niu, Jianjun,Zhang, Yongyou,et al. Preparation of His-tagged armored RNA phage particles as a control for real-time reverse transcription-PCR detection of severe acute respiratory syndrome coronavirus[J],2006.
APA	Cheng, Yangjian,Niu, Jianjun,Zhang, Yongyou,Huang, Jianwei,Li, Qingge,&李庆阁.(2006).Preparation of His-tagged armored RNA phage particles as a control for real-time reverse transcription-PCR detection of severe acute respiratory syndrome coronavirus..
MLA	Cheng, Yangjian,et al."Preparation of His-tagged armored RNA phage particles as a control for real-time reverse transcription-PCR detection of severe acute respiratory syndrome coronavirus".(2006).

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