QQ客服 官方微博 反馈留言

	Stain efficiency and MALDI-TOF MS compatibility of seven visible staining procedures
	Tian, Hong-yu（Chinese Acad Sci） ; gao, Xia（Chinese Acad Sci） ; xu, Gen-Jun（Chinese Acad Sci） ; zhao, Fu-Kun（Chinese Acad Sci） ; Lin, Jian-feng ; Chen, Qing-xi ; Chen QX(陈清西) ; yu, Mei-Lan（Zhejiang Sci Tech Univ）
	2008
关键词	mass spectrometry proteomics silver stain two-dimensional gel electrophoresis
英文摘要	Visible stain is still the most popular protein staining method used in proteomic approaches. However, most published data have been derived from comparisons between visible dyes and fluorescent dyes. In this work, we have focused on seven widely used visible staining procedures-Neuhoff CCB, blue silver, and five silver stains (LKB SN, He SN, Yan SN, Vorum SN, and Blum SN)-and studied their stain efficiencies and MALDI-TOF MS compatibilities on 1-D and 2-D PAGE. It was concluded that blue silver is slightly better in terms of stain efficiency than Neuhoff CCB, but it presented worse MS compatibility. Neuhoff CCB presented better MS compatibility and superior linearity but worse sensitivity than silver stains. Among the five silvering procedures, He SN showed the best MS compatibility and a reasonable staining efficiency; Yan SN lowered the chances of obtaining the protein identity by PMF but gave the best stain efficiency; Vorum SN gave a very clear background and a great contrast, while Blum SN was the worst in this respect. The implications of these results for the selection of a convenient stain are discussed according to specific objectives as well as practical aspects.
语种	英语
出版者	SPRINGER HEIDELBERG
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1007/s00216-008-1910-6]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Tian, Hong-yu,gao, Xia,xu, Gen-Jun,et al. Stain efficiency and MALDI-TOF MS compatibility of seven visible staining procedures[J],2008.
APA	Tian, Hong-yu.,gao, Xia.,xu, Gen-Jun.,zhao, Fu-Kun.,Lin, Jian-feng.,...&yu, Mei-Lan.(2008).Stain efficiency and MALDI-TOF MS compatibility of seven visible staining procedures..
MLA	Tian, Hong-yu,et al."Stain efficiency and MALDI-TOF MS compatibility of seven visible staining procedures".(2008).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。