QQ客服 官方微博 反馈留言

	Leucine Aminopeptidase from Red Sea Bream (Pagrus major) Skeletal Muscle: Purification, Characterization, Cellular Location, and Tissue Distribution
	Su, Wen-Jin ; Su WJ(苏文金) ; Wu, Guo-Ping ; Cao, Min-Jie（Biol Engn, Xiamen） ; Liu, Bing-Xin（Biol Engn, Xiamen） ; Chen, Yan（Jimei Univ）
	2008
关键词	Red sea bream leucine aminopeptidase purification characterization Western blot
英文摘要	A leucine aminopeptidase was purified for the first time from marine fish red sea bream (Pagrus major skeletal muscle to homogeneity with 4850-fold and a yield of 7.4%. The purification procedure consisted of ammonium sulfate fractionation and chromatographies including DEAE-Sephacel, Sephacryl S-200, hydroxyapatite, and phenyl-Sepharose. The enzyme was approximately 96 kDa as estimated by SIDS-PAGE and gel filtration and preferentially hydrolyzed substrate Leu-MCA. The enzymatic activity was optimal at 45 degrees C and pH 7.5. The K-m and k(cat) values of the enzyme for Leu-MCA were 1,55 mu M and 26.4 S-1 at 37 degrees C, respectively. Activation energy (E-a) of the enzyme was 59.6 kJ M-1. The enzyme was specifically inhibited by metal-chelating agents, and Zn2+ and (or) Mn2+ seemed to be its metal cofactor(s). In addition, bestatin strongly inhibited its activity, and K-i was 1.44 mu M. Using a highly specific polyclonal antibody, the location of enzyme was demonstrated intracellularly and distributed in different tissues.; National Natural Scientific Foundation of China [30571450]; Natural Scientific Foundation of Fujian Province [2006J0368]; Foundation for Innovative Research Team of Jimei University [2006A002]
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1021/jf801477r]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Su, Wen-Jin,Su WJ,Wu, Guo-Ping,et al. Leucine Aminopeptidase from Red Sea Bream (Pagrus major) Skeletal Muscle: Purification, Characterization, Cellular Location, and Tissue Distribution[J],2008.
APA	Su, Wen-Jin,苏文金,Wu, Guo-Ping,Cao, Min-Jie,Liu, Bing-Xin,&Chen, Yan.(2008).Leucine Aminopeptidase from Red Sea Bream (Pagrus major) Skeletal Muscle: Purification, Characterization, Cellular Location, and Tissue Distribution..
MLA	Su, Wen-Jin,et al."Leucine Aminopeptidase from Red Sea Bream (Pagrus major) Skeletal Muscle: Purification, Characterization, Cellular Location, and Tissue Distribution".(2008).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。