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	Evaluation of a rapid test for detection of H5N1 avian influenza virus
	Chen, Yixin ; Chen YX(陈毅歆) ; Zhang, Jun ; Zhang J(张军) ; Ge, Shenpiang ; Ge SX(葛胜祥) ; Luo, Haifeng ; Xu, Feihai ; Zheng, Qingbing ; Xia, Ningshao ; Xia NS(夏宁邵) ; Fan, Xiaohui（Univ Hong Kong） ; Chen, Honglin（Univ Hong Kong） ; Guan, Yi（Univ Hong Kong）
	2008
关键词	Avian influenza virus H5N1 Hemagglutinin Monoclonal antibody Point of care Dot ELISA
英文摘要	The performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was <= 0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.; Chinese National Project of Prevention and Treatment of Avian Influenza [2004BA519A73]; Chinese National Science and Technology Program [2006BA1011306]; Chinese National Natural and Science Foundation [30640017]; Science and Technology Project of Fujian Province in China [2005Y020, 2004YZ01-1]; Xiamen Science & Technology Program [3502Z20073001]; Research Fund for Control of Infectious Diseases of the Hong Kong Government
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.jviromet.2008.08.013]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Chen, Yixin,Chen YX,Zhang, Jun,et al. Evaluation of a rapid test for detection of H5N1 avian influenza virus[J],2008.
APA	Chen, Yixin.,陈毅歆.,Zhang, Jun.,张军.,Ge, Shenpiang.,...&Guan, Yi.(2008).Evaluation of a rapid test for detection of H5N1 avian influenza virus..
MLA	Chen, Yixin,et al."Evaluation of a rapid test for detection of H5N1 avian influenza virus".(2008).

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