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	Inhibitory Kinetics of beta-N-Acetyl-D-glucosaminidase from Prawn (Litopenaeus vannamei) by Zinc Ion
	Xie, Xiao-Lan ; Huang, Qian-Sheng ; Gong, Min ; Du, Juan ; Chen, Qing-Xi ; Chen QX(陈清西) ; Yang, Yi（ E China Univ Sci & Technol）
	2009
关键词	beta-N-acetyl-D-glucosaminidase Litopenaeus vannamei kinetics zinc ions inhibition
英文摘要	Prawn (Litopenaeus vannamei) beta-N-acetyl-D-glucosaminidase (NAGase, EC 3.2.1.52) is involved in the digestion and molting, processes. Zinc is one of the most important metals often found in the pollutant. In this article, the effects of Zn2+ on prawn NAGase activity for the hydrolysis of pNP-NAG have been investigated. The results showed that Zn2+ could reversibly and noncompetitively inhibit the enzyme activity at appropriate concentrations and its IC50 value was estimated to be 6.00 +/- 0.25 mM. The inhibition model was set up, and the inhibition kinetics of the enzyme by Zn2+ has been studied using the kinetic method of the substrate reaction. The inhibition constant was determined to he 11.96 mM and the microscopic rate constants were also determined for inactivation and reactivation. The rate constant of the inactivation (k(+0)) is much larger than that of the reactivation (k(-0)). Therefore, when the Zn2+ concentration is sufficiently large, the enzyme is completely inactivated. On increasing the concentration of Zn2+, the fluorescence emission peak and the UV absorbance peak are not position shifted, but the intensity decreased, indicating that the conformation of Zn2+-bound inactive NAGase is stable and different front that of native NAGase. We presumed that Zn2+ made changes in the activity and conformation of prawn NAGase by binding with the histidine or cysteine residues of the enzyme. (c) 2008 IUBMB; Natural Science Foundation of China [30500102]; Science and Technology Foundation of Fujian Province [2006N0067]; China Postdoctoral Science Foundation [20070410806]
语种	英语
出版者	JOHN WILEY & SONS INC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1002/iub.140]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Xie, Xiao-Lan,Huang, Qian-Sheng,Gong, Min,et al. Inhibitory Kinetics of beta-N-Acetyl-D-glucosaminidase from Prawn (Litopenaeus vannamei) by Zinc Ion[J],2009.
APA	Xie, Xiao-Lan.,Huang, Qian-Sheng.,Gong, Min.,Du, Juan.,Chen, Qing-Xi.,...&Yang, Yi.(2009).Inhibitory Kinetics of beta-N-Acetyl-D-glucosaminidase from Prawn (Litopenaeus vannamei) by Zinc Ion..
MLA	Xie, Xiao-Lan,et al."Inhibitory Kinetics of beta-N-Acetyl-D-glucosaminidase from Prawn (Litopenaeus vannamei) by Zinc Ion".(2009).

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