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	Characterization and expression of AmphiBMP3/3b gene in amphioxus Branchiostoma japonicum
	Sun, Yi ; Zhang, Qiu-Jin ; Zhong, Jing ; Wang, Yi-Quan ; Wang YQ(王义权)
	2010-02
关键词	amphioxus BMP3 3b gene developmental embryo evolution gene expression pattern
英文摘要	Bone morphogenetic proteins (BMPs) are responsible for regulating embryo development and tissue homeostasis beyond osteogenesis. However, the precise biological roles of BMP3 and BMP3b remain obscure to a certain extent. In the present study, we cloned an orthologous gene (AmphiBMP3/3b) from amphioxus (Branchiostoma japonicum) and found its exon/intron organization is highly conserved. Further, in situ hybridization revealed that the gene was strongly expressed in the dorsal neural plate of the embryos. The gene also appeared in Hatschek's left diverticulum, neural tube, preoral ciliated pit and gill slit of larvae, and adult tissues including ovary, neural tube and notochordal sheath. Additionally, real-time quantitative polymerase chain reaction (RTqPCR) analysis revealed that the expression displayed two peaks at gastrula and juvenile stages. These results indicated that AmphiBMP3/3b, a sole orthologue of vertebrate BMP3 and BMP3b, might antagonize ventralizing BMP2 orthologous signaling in embryonic development, play a role in the evolutionary precursors of adenohypophysis, as well as act in female ovary physiology in adult.; National Natural Science Foundation of China [30830023]; National High Technology Research and Development Program of China (863 Program) [2008AA092602]; Ministry of Education, China [20070384041]
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1111/j.1440-169X.2009.01150.]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Sun, Yi,Zhang, Qiu-Jin,Zhong, Jing,et al. Characterization and expression of AmphiBMP3/3b gene in amphioxus Branchiostoma japonicum[J],2010.
APA	Sun, Yi,Zhang, Qiu-Jin,Zhong, Jing,Wang, Yi-Quan,&王义权.(2010).Characterization and expression of AmphiBMP3/3b gene in amphioxus Branchiostoma japonicum..
MLA	Sun, Yi,et al."Characterization and expression of AmphiBMP3/3b gene in amphioxus Branchiostoma japonicum".(2010).

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