REGENERATION OF PLANTLETS AND TETRAPLOIDY INDUCTION IN PSEUDOSTELLARIA HETEROPHYLLA

CORC > 厦门大学 > 生命科学－已发表论文

	REGENERATION OF PLANTLETS AND TETRAPLOIDY INDUCTION IN PSEUDOSTELLARIA HETEROPHYLLA
	Ye, Zuyun ; Tian, Hui Qiao ; Tian HQ(田惠桥) ; Wang, Ya Ying
	2010
关键词	Callus embryonic calli Pseudostellaria heterophylla regeneration tetraploidy
英文摘要	This study was aimed at developing an efficient protocol for regeneration of Pseudostellaria heterophylla plantlets and induction of polyploidy. Calli of P. heterophylla (Miq) from stems, leaves and buds as explants could not differentiate into plantlets. However, Young embryo segments used as primary explants produced embryonic calli on MS medium containing 5.0 mg/L 2,4-D and 0.5 mg/L KT. After the embryonic calli with granular protuberances were transferred to MS medium containing 0.5 mg/L BA, they developed shoots and then rooted to form plantlets. Polyploidy was induced when embryonic calli were placed in liquid MS medium containing 0.5% colchicine for 4 days, followed by culturing in solid medium to induce differentiation. Polyploidy was identified by the number of chromosomes and the size of plantlet stomata. The tetraploid plantlets produced larger root tubers than the diploid plantlets.; National Natural Science Foundation of China [30670126]; Xiamen Science Item [3502Z20084037]
语种	英语
出版者	POLSKA AKAD NAUK
内容类型	期刊论文
源URL	[http://www.ib.uj.edu.pl/abc/pdf/51_2/02_ye.pdf]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ye, Zuyun,Tian, Hui Qiao,Tian HQ,et al. REGENERATION OF PLANTLETS AND TETRAPLOIDY INDUCTION IN PSEUDOSTELLARIA HETEROPHYLLA[J],2010.
APA	Ye, Zuyun,Tian, Hui Qiao,田惠桥,&Wang, Ya Ying.(2010).REGENERATION OF PLANTLETS AND TETRAPLOIDY INDUCTION IN PSEUDOSTELLARIA HETEROPHYLLA..
MLA	Ye, Zuyun,et al."REGENERATION OF PLANTLETS AND TETRAPLOIDY INDUCTION IN PSEUDOSTELLARIA HETEROPHYLLA".(2010).