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	Increased biological hydrogen production by deletion of hydrogen-uptake system in photosynthetic bacteria
	Liang, Yi ; Wu, Xiaobing ; Gan, Lihui ; Xu, Huijuan ; Hu, Zhong ; Long, Minnan ; Long MN(龙敏南)
	2009
关键词	Allochromatium vinosum Membrane-bound hydrogenase Gene cloning Deletion of hydrogen-uptake system Biological hydrogen production
英文摘要	Hydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H-2-uptake hydrogenase and H-2-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vinosum was cloned and sequenced. It consist of two structural genes (hydS, hydL) and two intergenic genes (isp1, isp2), which are therefore organized as hydS-isp1-isp2-hydL. This is different from the arrangement of other typical hydrogenase gene clusters. A deletion mutant-strain Phi hydSL, tacking isp1, isp2, partial hydS and hydL genes, was constructed by marker-exchange mutagenesis. Under dark fermentative conditions, the hydrogen production yield by this mutant increased by 62%. The result suggests that the disruption of MBH could greatly improve the hydrogen production in the cells by decreasing the hydrogen uptake. (C) 2009 Elsevier GmbH. All rights reserved.; National 863 project of China [2006AA05Z111]; NSFC National Foundation for Fostering Talents of Basic Science [J0630649, 30470395]
语种	英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.micres.2009.03.001]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liang, Yi,Wu, Xiaobing,Gan, Lihui,et al. Increased biological hydrogen production by deletion of hydrogen-uptake system in photosynthetic bacteria[J],2009.
APA	Liang, Yi.,Wu, Xiaobing.,Gan, Lihui.,Xu, Huijuan.,Hu, Zhong.,...&龙敏南.(2009).Increased biological hydrogen production by deletion of hydrogen-uptake system in photosynthetic bacteria..
MLA	Liang, Yi,et al."Increased biological hydrogen production by deletion of hydrogen-uptake system in photosynthetic bacteria".(2009).

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