| 抗人DcR3单克隆抗体的制备、鉴定及应用; Preparation,characterization,and application of monoclonal antibodies against human decoy receptor 3 | |
| 刘瑞振 ; 张长弓 ; 何琼 ; 苏金华 ; 陈彩霞 ; 陈福 ; 庄国洪 | |
| 2009-01 | |
| 关键词 | DcR3 单克隆抗体 制备 monoclonal antibody preparation |
| 英文摘要 | 目的制备抗DcR3单克隆抗体(mAb),鉴定其生物学特性,并应用于ELISA、Western blot、Flowcytometry(FCM)检测。方法以纯化的可溶性DcR3(sDcR3)免疫Balb/c小鼠,采用杂交瘤技术制备抗DcR3 mAb。用Ig亚类ELISA试剂盒鉴定抗DcR3mAb的亚类。用ELISA方法测定抗DcR3 mAb与sDcR3结合的特性,SDS-PAGE鉴定抗DcR3 mAb与SW480细胞上清中DcR3结合的特性,以鉴定mAb的特性。用间接ELISA法检测腹水mAb的效价、亲和常数并进行表位分析。Western blot检测mAb的特异性及应用,并用所获抗DcR3单克隆抗体(mAb)通过流式细胞仪检测肿瘤细胞表面DcR3的表达水平。结果获得4株可分泌DcR3 mAb的杂交瘤细胞系ZZ-393、ZZ-394、ZZ-151和ZZ-268。其中DcR3 mAb ZZ-268(下文简称为ZZ-268)的Ig亚类为IgG1(κ型);腹水效价为1×10-5;亲和常数为1.28×109水平;ZZ-268和ZZ-151可识别与其他2种抗体不同的抗原表位;Western blot证实,获得的ZZ-268... 【英文摘要】 Objective To prepare monoclonal antibodies mAb against DcR3,characterize their biological properties and apply them to ELISA,Flow cytometry,and Western blot detection.Methods Balb/c mice were immunized with soluble DcR3(sDcR3) for preparing mAb by using hybridoma technique.Ig subclass and specificity of mAbs were analyzed by ELISA and SDS-PAGE,respectively.The titres,relative affinity,and epitopes of mAbs in ascitic fluid were determined by indirect ELISA.The specificity and application of mAb were detected...; 厦门大学科研启动资金(Z03103);; 厦门市科技计划项目(3502Z20083008)资助 |
| 语种 | 中文 |
| 出版者 | 第三军医大学;中国免疫学会 |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/6234]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 刘瑞振,张长弓,何琼,等. 抗人DcR3单克隆抗体的制备、鉴定及应用, Preparation,characterization,and application of monoclonal antibodies against human decoy receptor 3[J],2009. |
| APA | 刘瑞振.,张长弓.,何琼.,苏金华.,陈彩霞.,...&庄国洪.(2009).抗人DcR3单克隆抗体的制备、鉴定及应用.. |
| MLA | 刘瑞振,et al."抗人DcR3单克隆抗体的制备、鉴定及应用".(2009). |
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