Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

	Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry
	Xu, SY ; Pan, CS ; Hu, LG ; Zhang, Y ; Guo, Z ; Li, X ; Zou, HF
刊名	electrophoresis
	2004-11-01
卷号	25 期号:21-22 页码:3669-3676
关键词	desorption/ionization on silicon enzyme inhibitor immobilized enzyme matrix assisted laser desorption/ioniozation-time of flight-mass spectrometry miniaturization peptide mapping
产权排序	1;1
英文摘要	desorption/ionization on silicon mass spectrometry (dios-ms) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. this technique has a relatively high tolerance for contaminants commonly found in biological samples. dios-ms has been applied to determine the activity of immobilized enzymes on the porous silicon surface. enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. it is demonstrated that this method can be applied to the screening of enzyme inhibitors. furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-ms has been developed.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	protein identification ; peptide ; proteomics ; platform
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000225679000016
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/81995]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, SY,Pan, CS,Hu, LG,et al. Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry[J]. electrophoresis,2004,25(21-22):3669-3676.
APA	Xu, SY.,Pan, CS.,Hu, LG.,Zhang, Y.,Guo, Z.,...&Zou, HF.(2004).Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry.electrophoresis,25(21-22),3669-3676.
MLA	Xu, SY,et al."Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry".electrophoresis 25.21-22(2004):3669-3676.