Trapping yeast cells on PDMS micropillar array | |
Zhang, Wei ; Zhang, Bo ; Zhang, Yulong ; Han, Da ; Zhou, Yongliang ; Zhang B(张博) ; Zhang YL(张玉龙) ; Zhou YL(周勇亮) | |
2012 | |
关键词 | Adhesion Cell engineering Cells Cytology Manufacture Microchannels Surfaces Yeast |
英文摘要 | Conference Name:3rd International Conference on Manufacturing Science and Engineering, ICMSE 2012. Conference Address: Xiamen, China. Time:March 27, 2012 - March 29, 2012.; Fujian University of Technology; Xiamen University; Fuzhou University; Huaqiao University; University of Wollongong; A selectively yeast cell trapping and arraying method was presented, utilizing PDMS micropillar array combining with chemical adhesion. Yeast cells were trapped on the top of micropillar array while swept on the flat area for the pinning of liquid/surface contact line on the micropillars and moving on the flat surface. And the modification of poly-L-lysine on the yeast cells improved the immobilization of cells on the surface of PDMS surface. Both of simulation and experiment results shows that by adjusting the diameter of micropillars, the number of yeast cells on each pillars could be controlled. Single cell array was formed with a 8.3 渭m micropillar array, and majority yeast numbers of 3, 4,5 was got for the 13.7 渭m, 18.0 渭m and 18.8 渭m micropillar array. 漏 (2012) Trans Tech Publications. |
语种 | 英语 |
出处 | http://dx.doi.org/10.4028/www.scientific.net/AMR.476-478.2096 |
出版者 | Trans Tech Publications |
内容类型 | 其他 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/85418] |
专题 | 化学化工-会议论文 |
推荐引用方式 GB/T 7714 | Zhang, Wei,Zhang, Bo,Zhang, Yulong,et al. Trapping yeast cells on PDMS micropillar array. 2012-01-01. |
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