核苷酸、聚核苷酸和核酸猝灭Tb--(3+)-钛铁试剂络合物荧光的机理研究; Studies on the Mechanism of Fluorescence Quenching of Tb--(3+)-Tiron Complex by Nucleotides, Polynucleotides and Nucleic Acids | |
赵一兵 ; 王冬媛 ; 郭祥群 ; 许金钩 | |
1997 | |
关键词 | Tb--(3+)-钛铁试剂络合物 核苷酸 聚核苷酸 核酸 荧光猝灭机理 Tb--(3+)-Trion complex Nucleotides Polynucleotides Nucleic acids Mechanisin of Fluorescence quenching |
英文摘要 | 研究了核苷酸、聚核苷酸和核酸对Tb3+-钛铁试剂(Tr)络合物的荧光碎灭机理,认为荧光猝灭过程是核苷酸、聚核苷酸和核酸分子中的磷酸基组分与Tr竞争Tb3+离子,生成实验条件下无荧光的二元络合物的静态猝灭过程;用Tb3+-Tr络合物荧光探针研究dnA嵌入剂和金属离子与dnA相互作用的实验结果说明这一机理是合理的.; The mechanism of Fluorescence quenching of Tb3+-Trion complex by nucleotides,polynucleotides and nucleic acids has been studied.It was considered that it is a static quenching which is based on the phosphate moieties of nucleotides, polynucleotides and nucleic acids conpeting the Tb3+ ion with Tiron and Forming non-Fluorescent binary complex under the optimal conditions.The experimental results of nucleic acids reacting with intercalating reagent of DNA or metal ion using Tb3+-Trion complex as a Fluorescence probe indicated that the mechanism is reasonable.; 国家教育委员会博士点基金 |
语种 | zh_CN |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/107275] |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | 赵一兵,王冬媛,郭祥群,等. 核苷酸、聚核苷酸和核酸猝灭Tb--(3+)-钛铁试剂络合物荧光的机理研究, Studies on the Mechanism of Fluorescence Quenching of Tb--(3+)-Tiron Complex by Nucleotides, Polynucleotides and Nucleic Acids[J],1997. |
APA | 赵一兵,王冬媛,郭祥群,&许金钩.(1997).核苷酸、聚核苷酸和核酸猝灭Tb--(3+)-钛铁试剂络合物荧光的机理研究.. |
MLA | 赵一兵,et al."核苷酸、聚核苷酸和核酸猝灭Tb--(3+)-钛铁试剂络合物荧光的机理研究".(1997). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论