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	山萘酚对脂多糖诱导RAW264.7细胞中COX-2和iNOS表达及产物生成的影响; The effect of kaempferol on COX-2and iNOS expression and generated production in LPS-induced RAW 264.7 cells
	陈美珺 ; 金宏伟 ; 陈振胜 ; 黄回滨 ; 梁统 ; 周克元
	2013-12-20
关键词	山萘酚 环氧合酶-2 诱导型一氧化氮合酶 前列腺素E2 一氧化氮 kaempferol cycloxygenase-2 inducible nitric oxide synthase prostaglandins E2 nitric oxide
英文摘要	目的探讨山萘酚对脂多糖(lIPOPOlySACCHArIdES,lPS)诱导rAW 264.7细胞COX-2及InOS表达的影响。方法四氮唑盐法(MOnOTE-TrAzOlIuM TEST,MTT)检测山奈酚对rAW264.7细胞生长增殖的影响,放射免疫测定法(rIA)检测山萘酚对PgE2和nO生成的影响,免疫印迹法(WESTErn blOTTIng)检测COX-2及InOS蛋白的表达。结果山萘酚抑制lPS诱导的rAW 264.7细胞PgE2和nO的生成,同时下调lPS诱导的rAW 264.7细胞COX-2及InOS蛋白的表达。结论山萘酚抑制2个诱导酶COX-2和InOS的表达,从而减少炎性产物PgE2和nO的生成,这可能是山萘酚抗炎的机制之一。; Objective To study the effect of kaempferol on COX-2and iNOS expression in LPS-induced RAW 264.7cells.Methods The effects of kaempferol on the proliferation in RAW264.7cells were evaluated by monote-trazolium test assay(MTT);the effect o f kaempferol on the product of PGE2and NO in RAW264.7cells was measured by radioimmunoassay(RIA);the effects of kaempferol on the expressions of COX-2and iNOS protein in RAW264.7cells were analyzed by Western blot.Results Kaempferol inhibited the LPS-induced PGE2and NO synthesis in RAW 264.7cells.The protein expression of COX-2and iNOS in RAW264.7cells were also decreased by kaempferol.Conclusions The results show that kaempferol reduces the augmented biosynthesis of PGE2and NO by inhibiting the protein expression of COX-2and iNOS in RAW264.7cells.The finding may partly explain the anti-inflammatory effect of kaempferol.; 广东省社会发展领域科技计划项目(53025); 广东省重点扶持学科项目(GX0307)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/106868]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	陈美珺,金宏伟,陈振胜,等. 山萘酚对脂多糖诱导RAW264.7细胞中COX-2和iNOS表达及产物生成的影响, The effect of kaempferol on COX-2and iNOS expression and generated production in LPS-induced RAW 264.7 cells[J],2013.
APA	陈美珺,金宏伟,陈振胜,黄回滨,梁统,&周克元.(2013).山萘酚对脂多糖诱导RAW264.7细胞中COX-2和iNOS表达及产物生成的影响..
MLA	陈美珺,et al."山萘酚对脂多糖诱导RAW264.7细胞中COX-2和iNOS表达及产物生成的影响".(2013).

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