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	链霉菌G-HD-4产黑色素发酵条件的优化; Optimal Fermentation Conditions for Melanin Production by Streptomyces G-HD-4
	郑晨娜 ; 罗婉妹 ; 陈慧勤 ; 吴晓岚 ; 方柏山
	2010
关键词	链霉菌G-HD-4 黑色素 发酵 均匀设计 Streptomyces G-HD-4 melanin fermentation uniform-design
英文摘要	为提高链霉菌g-Hd-4的黑色素产生量,以l-酪氨酸为底物,对该菌种产黑色素的工艺条件进行研究,通过单因素试验和均匀设计试验,得到最佳产黑色素的发酵条件为:马铃薯15g/100Ml、乳糖2.5g/100Ml、干酪素2.07g/100Ml、MgSO40.11g/100Ml、kH2PO40.25g/100Ml、l-酪氨酸0.25g/100Ml、以接种量为6%(体积分数),培养基起始PH值为6.0,装液量为25Ml,28℃,150r/MIn振荡培养5d,黑色素产量可达(13.4±0.05)g/l,比在基础培养基中的黑色素产量(5.78g/l)提高约2.3倍。; In order to improve melanin production by Streptomyces G-HD-4 fermentation, the optimal conditions for fermenting L-tyrosine by this strain were investigate by single factor and uniform design methods.The optimal medium compositions (on the basis of 100 mL medium) were 15 g potato, 2.5 g lactose, 2.07 g casein, 0.25 g KH2PO4, 0.11 g MgSO4, and 0.25 g L-tyrosine.The optimal fermentation process was that 25 mL of medium was contained in a 250 mL flask for 5-day fermentation at initial pH 6.0, 28 ℃ and 150 r/min shaking speed.As a result, the maximum melanin yield reached up to (13.4 ± 0.05) g/L, approximately 2.3 times higher than that obtained using preoptimization fermentation medium.
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/106381]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	郑晨娜,罗婉妹,陈慧勤,等. 链霉菌G-HD-4产黑色素发酵条件的优化, Optimal Fermentation Conditions for Melanin Production by Streptomyces G-HD-4[J],2010.
APA	郑晨娜,罗婉妹,陈慧勤,吴晓岚,&方柏山.(2010).链霉菌G-HD-4产黑色素发酵条件的优化..
MLA	郑晨娜,et al."链霉菌G-HD-4产黑色素发酵条件的优化".(2010).

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