Progress towards bioorthogonal catalysis with organometallic compounds

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	Progress towards bioorthogonal catalysis with organometallic compounds
	Vlker, Timo ; Dempwolff, Felix ; Graumann, Peter L. ; Meggers, ; ERIC MEGGERS
刊名	http://dx.doi.org/10.1002/anie.201404547
	2014
关键词	Catalysts Cell death Fluorescence Organometallics Reaction kinetics Ruthenium compounds
英文摘要	The catalysis of bioorthogonal transformations inside living organisms is a formidable challenge - yet bears great potential for future applications in chemical biology and medicinal chemistry. We herein disclose highly active organometallic ruthenium complexes for bioorthogonal catalysis under biologically relevant conditions and inside living cells. The catalysts uncage allyl carbamate protected amines with unprecedented high turnover numbers of up to 270 cycles in the presence of water, air, and millimolar concentrations of thiols. By live-cell imaging of HeLa cells and with the aid of a caged fluorescent probe we could reveal a rapid development of intense fluorescence within the cellular cytoplasm and therefore support the proposed bioorthogonality of the catalysts. In addition, to illustrate the manifold applications of bioorthogonal catalysis, we developed a method for catalytic in-cell activation of a caged anticancer drug, which efficiently induced apoptosis in HeLa cells. ? 2014 Wiley-VCH Verlag GmbH & Co. KGaA.
语种	英语
出版者	Wiley-VCH Verlag
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89686]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Vlker, Timo,Dempwolff, Felix,Graumann, Peter L.,et al. Progress towards bioorthogonal catalysis with organometallic compounds[J]. http://dx.doi.org/10.1002/anie.201404547,2014.
APA	Vlker, Timo,Dempwolff, Felix,Graumann, Peter L.,Meggers,,&ERIC MEGGERS.(2014).Progress towards bioorthogonal catalysis with organometallic compounds.http://dx.doi.org/10.1002/anie.201404547.
MLA	Vlker, Timo,et al."Progress towards bioorthogonal catalysis with organometallic compounds".http://dx.doi.org/10.1002/anie.201404547 (2014).