3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations

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	3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations
	Cui, P.X ; Lian, F.L. ; Wang, ; Wen, Yi ; Chu, W.S ; Zhao, H.F. ; Zhang, S. ; Li, J. ; Lin, D.H. ; Wu, Z.Y. ; Zhang S(张飒) ; Lin DH(林东海)
刊名	http://dx.doi.org/10.1016/j.radphyschem.2013.01.043
	2014
关键词	Amino acids Mammals Proteins Scattering Three dimensional X ray absorption near edge structure spectroscopy
英文摘要	Prion-related protein (PrP), a cell-surface copper-binding glycoprotein, is considered to be responsible for a number of transmissible spongiform encephalopathies (TSEs). The structural conversion of PrP from the normal cellular isoform (PrPC) to the post-translationally modified form (PrPSc) is thought to be relevant to Cu2+ binding to histidine residues. Rabbits are one of the few mammalian species that appear to be resistant to TSEs, because of the structural characteristics of the rabbit prion protein (RaPrPC) itself. Here we determined the three-dimensional local structure around the C-terminal high-affinity copper-binding sites using X-ray absorption near-edge structure combined with ab initio calculations in the framework of the multiple-scattering (MS) theory. Result shows that two amino acid resides, Gln97 and Met108, and two histidine residues, His95 and His110, are involved in binding this copper(II) ion. It might help us understand the roles of copper in prion conformation conversions, and the molecular mechanisms of prion-involved diseases. ? 2013 Elsevier Ltd.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89681]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Cui, P.X,Lian, F.L.,Wang,,et al. 3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations[J]. http://dx.doi.org/10.1016/j.radphyschem.2013.01.043,2014.
APA	Cui, P.X.,Lian, F.L..,Wang,.,Wen, Yi.,Chu, W.S.,...&林东海.(2014).3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations.http://dx.doi.org/10.1016/j.radphyschem.2013.01.043.
MLA	Cui, P.X,et al."3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations".http://dx.doi.org/10.1016/j.radphyschem.2013.01.043 (2014).