Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns

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	Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns
	Han, Jing ; Ye, Linquan ; Xu, Lingjia ; Zhou, Zhuoheng ; Gao, Fan ; Xiao, Zhiliang ; Wang, Qiuquan ; Zhang, Bo ; Wang QQ(王秋泉) ; Zhang B(张博)
刊名	http://dx.doi.org/10.1016/j.aca.2014.09.006
	2014
关键词	Column chromatography Liquids Molecular biology Particles (particulate matter)
英文摘要	Single shot proteomics is a promising approach to high throughput proteomics analysis. In this strategy, long capillary columns are needed to perform long and shallow gradients to achieve high peak capacity and good peak width for informative mass spectrometric detection. Herein, we report that meter long capillary columns, packed with 5. μm particulate material, can be facilely fabricated based on single particle fritting technology. The long columns could reliably generate high peak capacities of 800 in 10. h long gradients for protein digest separations. The operation was within the pressure range (40. MPa) of the most widely used normal pressure nanoLC systems. Due to the excellent life time (>100 injections) and inter-column performance consistency, the meter long capillary columns reported here should be of practical usefulness in single shot proteomics without the need for ultra-high pressure instrumentation.
语种	英语
出版者	Elsevier
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89637]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Han, Jing,Ye, Linquan,Xu, Lingjia,et al. Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns[J]. http://dx.doi.org/10.1016/j.aca.2014.09.006,2014.
APA	Han, Jing.,Ye, Linquan.,Xu, Lingjia.,Zhou, Zhuoheng.,Gao, Fan.,...&张博.(2014).Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns.http://dx.doi.org/10.1016/j.aca.2014.09.006.
MLA	Han, Jing,et al."Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns".http://dx.doi.org/10.1016/j.aca.2014.09.006 (2014).