Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance

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	Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance
	Chen, Xiaomei ; Tian, Xiaotian ; Su, Bingyuan ; Huang, Zhiyong ; Chen, Xi ; Oyama, Munetaka ; Chen X(陈曦)
刊名	http://dx.doi.org/10.1039/c3dt53316f
	2014
关键词	GOLD NANOPARTICLES COLORIMETRIC DETECTION CATALYTIC-ACTIVITY CARBON NANOTUBES GLUCOSE INTERFACE OXIDATION METHANOL GROWTH ENZYME
英文摘要	Japan Society for Promotion of Science (JSPS); National Natural Science Foundation of China [21305050]; Special Foundation for Young researchers of Health Department of Fujian [2013-2-106]; Scientific Research Foundation of Shangda Li, Jimei University [ZC2013005]; JSPS KAKENHI [2402335, 24550100]; In this paper, Au nanoparticles (AuNPs) have been homogeneously deposited on citrate-functionalized graphene nanosheets (Cit-GNs) by a simple one-pot reducing method. The morphology and composition of the thus-prepared AuNPs/Cit-GNs were characterized by transmission electron microscopy (TEM), high resolution TEM, energy dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The results showed that the AuNPs with a uniform size are well dispersed on the surface of the Cit-GNs. Significantly, the as-prepared AuNPs/Cit-GNs possess intrinsic peroxidase-like activity, which can catalyze the oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) to develop a blue color in aqueous solution. The catalysis was in accordance with Michaelis-Menten kinetics and the AuNPs/Cit-GNs showed a strong affinity for both H2O2 and TMB. Moreover, by comparing with Cit-AuNPs, AuNPs/GNs and AuNPs/PVP-GNs, the AuNPs/Cit-GNs composite exhibits a higher catalytic ability with a lower Michaelis constant (Km) value, suggesting that the GNs with a large surface area and the citrate ions with more carboxyl groups around the AuNPs can greatly enhance the peroxidase-like activity of AuNPs/Cit-GNs. Taking the advantages of the high catalytic activity, the good stability and the low cost, the novel AuNPs/Cit-GNs represent a promising candidate as an enzyme mimic and may find a wide range of new applications in biochemistry and biotechnology.
语种	英语
出版者	ROYAL SOC CHEMISTRY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89444]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Chen, Xiaomei,Tian, Xiaotian,Su, Bingyuan,et al. Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance[J]. http://dx.doi.org/10.1039/c3dt53316f,2014.
APA	Chen, Xiaomei.,Tian, Xiaotian.,Su, Bingyuan.,Huang, Zhiyong.,Chen, Xi.,...&陈曦.(2014).Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance.http://dx.doi.org/10.1039/c3dt53316f.
MLA	Chen, Xiaomei,et al."Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance".http://dx.doi.org/10.1039/c3dt53316f (2014).