A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells | |
Wang, Xiaojing ; Miao, Qingqing ; Song, Tingjie ; Yuan, Qingpan ; Gao, Jinhao ; Liang, Gaolin ; Gao JH(高锦豪) | |
刊名 | http://dx.doi.org/10.1039/c4an00410h |
2014 | |
关键词 | CU2+ IONS AQUEOUS-SOLUTION HIGH-SENSITIVITY AMINO-ACIDS SENSOR PROBE MECHANISM PRECONCENTRATION SPECTROMETRY RECOGNITION |
英文摘要 | National Natural Science Foundation of China [21175122, 91127036, 21375121]; Fundamental Research Funds for Central Universities [WK2060190018]; Herein, we report the development of a new fluorescent switch for sequential and selective sensing of Cu2+ and L-histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ- Gly-Gly-Gly-Lys (FITC) (1) exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates to Cu2+, causing its fluorescence emission to be quenched via photoinduced electron transfer. Interestingly, the as-formed 1-Cu2+ complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied for qualitatively and quantitatively sensing Cu2+ and L-His in vitro. Using this dual functional probe, we also sequentially imaged Cu2+ and L-His in living HepG2 cells. Our new probe 1 could be applied for not only environmental monitoring but also biomolecule detection in the near future. |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/89431] |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Wang, Xiaojing,Miao, Qingqing,Song, Tingjie,et al. A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells[J]. http://dx.doi.org/10.1039/c4an00410h,2014. |
APA | Wang, Xiaojing.,Miao, Qingqing.,Song, Tingjie.,Yuan, Qingpan.,Gao, Jinhao.,...&高锦豪.(2014).A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells.http://dx.doi.org/10.1039/c4an00410h. |
MLA | Wang, Xiaojing,et al."A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells".http://dx.doi.org/10.1039/c4an00410h (2014). |
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