CORC  > 厦门大学  > 化学化工-已发表论文
A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells
Wang, Xiaojing ; Miao, Qingqing ; Song, Tingjie ; Yuan, Qingpan ; Gao, Jinhao ; Liang, Gaolin ; Gao JH(高锦豪)
刊名http://dx.doi.org/10.1039/c4an00410h
2014
关键词CU2+ IONS AQUEOUS-SOLUTION HIGH-SENSITIVITY AMINO-ACIDS SENSOR PROBE MECHANISM PRECONCENTRATION SPECTROMETRY RECOGNITION
英文摘要National Natural Science Foundation of China [21175122, 91127036, 21375121]; Fundamental Research Funds for Central Universities [WK2060190018]; Herein, we report the development of a new fluorescent switch for sequential and selective sensing of Cu2+ and L-histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ- Gly-Gly-Gly-Lys (FITC) (1) exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates to Cu2+, causing its fluorescence emission to be quenched via photoinduced electron transfer. Interestingly, the as-formed 1-Cu2+ complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied for qualitatively and quantitatively sensing Cu2+ and L-His in vitro. Using this dual functional probe, we also sequentially imaged Cu2+ and L-His in living HepG2 cells. Our new probe 1 could be applied for not only environmental monitoring but also biomolecule detection in the near future.
语种英语
出版者ROYAL SOC CHEMISTRY
内容类型期刊论文
源URL[http://dspace.xmu.edu.cn/handle/2288/89431]  
专题化学化工-已发表论文
推荐引用方式
GB/T 7714
Wang, Xiaojing,Miao, Qingqing,Song, Tingjie,et al. A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells[J]. http://dx.doi.org/10.1039/c4an00410h,2014.
APA Wang, Xiaojing.,Miao, Qingqing.,Song, Tingjie.,Yuan, Qingpan.,Gao, Jinhao.,...&高锦豪.(2014).A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells.http://dx.doi.org/10.1039/c4an00410h.
MLA Wang, Xiaojing,et al."A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells".http://dx.doi.org/10.1039/c4an00410h (2014).
个性服务
查看访问统计
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。


©版权所有 ©2017 CSpace - Powered by CSpace