Toward rapid preparation of capillary columns for electrochromatography use

CORC > 厦门大学 > 化学化工－已发表论文

	Toward rapid preparation of capillary columns for electrochromatography use
	Liu, Qing ; Wang, Lin ; Zhou, Zhuoheng ; Wang, Qiuquan ; Yan, Lijuan ; Zhang, Bo ; Zhang B(张博)
刊名	http://dx.doi.org/10.1002/elps.201300503
	2014
关键词	LIQUID-CHROMATOGRAPHY PACKED CAPILLARIES PROTEOMICS FRITS PERFORMANCE TECHNOLOGY SEPARATION PEPTIDES PRESSURE PROTEINS
英文摘要	National Natural Science Foundation [21035006, 21005065]; Ministry of Education [20100121120006]; Fundamental Research Funds for Central Universities of China [2010121013, 2012121023]; Natural Science Foundation of Fujian Province [2010J05031, 2011J05029]; NFFTBS [J1310024]; CEC is a high performance electrodriving liquid phase separation technique. It does not need complex and sophisticated high pressure instrumentation for nanoflow driving. This is attractive for parallel multicolumn analysis. To this end, high throughput methods for column preparation are needed to support the use of multiple columns. In this study, we directly used CEC mobile phase solution as the packing solvent, and realized rapid preparation of capillary columns based on a single particle fritting technology. The method presented high preparation throughput compared with other reported methods based on various fritting technologies. The single particle fritting approach promoted column preparation throughput to 1 column/h, including all the fritting, packing and conditioning steps. The rapidly prepared columns showed consistently high efficiency of up to 150000 plates per meter, and usefulness in reversed phase CEC of neutral, charged and biomolecules. With standard peptides as the sample, excellent long term reproducibility (better than 0.8%RSD, ten days, for retention times) was observed.
语种	英语
出版者	WILEY-BLACKWELL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89371]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Liu, Qing,Wang, Lin,Zhou, Zhuoheng,et al. Toward rapid preparation of capillary columns for electrochromatography use[J]. http://dx.doi.org/10.1002/elps.201300503,2014.
APA	Liu, Qing.,Wang, Lin.,Zhou, Zhuoheng.,Wang, Qiuquan.,Yan, Lijuan.,...&张博.(2014).Toward rapid preparation of capillary columns for electrochromatography use.http://dx.doi.org/10.1002/elps.201300503.
MLA	Liu, Qing,et al."Toward rapid preparation of capillary columns for electrochromatography use".http://dx.doi.org/10.1002/elps.201300503 (2014).