Monoclonal Surface Display SELEX for Simple, Rapid, Efficient, and Cost-Effective Aptamer Enrichment and Identification

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	Monoclonal Surface Display SELEX for Simple, Rapid, Efficient, and Cost-Effective Aptamer Enrichment and Identification
	Zhu, Zhi ; Song, Yanling ; Li, Cong ; Zou, Yuan ; Zhu, Ling ; An, Yuan ; Yang, Chaoyong James ; Zhu Z(朱志) ; Yang CY(杨朝勇)
刊名	http://dx.doi.org/10.1021/ac501423g
	2014-06-17
关键词	CELL ADHESION MOLECULE DNA APTAMERS BIOANALYTICAL APPLICATIONS CAPILLARY-ELECTROPHORESIS CHEMICAL BIOLOGY SELECTION CANCER LIGANDS AMPLIFICATION EXPRESSION
英文摘要	National Basic Research Program of China [2010CB732402, 2013CB933703]; National Science Foundation of China [91313302, 21205100, 21275122, 21075104]; National Instrumentation Program [2011YQ03012412]; Natural Science Foundation of Fujian Province, China [2013J01061]; Fundamental Research Funds for the Central Universities [2012121025]; National Science Foundation for Distinguished Young Scholars of China [21325522]; A novel method, monoclonal surface display SELEX (MSD-SELEX), has been designed for simple, rapid, efficient, and cost-effective enrichment and identification of aptamers from a library of monoclonal DNA-displaying beads produced via highly parallel single-molecule emulsion PCR. The approach was successfully applied for the identification of high-affinity aptamers that bind specifically to different types of targets, including cancer biomarker protein EpCAM and small toxin molecule aflatoxin B-1. Compared to the conventional sequencing-chemical synthesis-screening work flow, MSD-SELEX avoids large-scale DNA sequencing, expensive and time-consuming DNA synthesis, and labor-intensive screening of large populations of candidates, thus offering a new approach for simple, rapid, efficient, and cost-effective aptamer identification for a wide variety of applications.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89269]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhu, Zhi,Song, Yanling,Li, Cong,et al. Monoclonal Surface Display SELEX for Simple, Rapid, Efficient, and Cost-Effective Aptamer Enrichment and Identification[J]. http://dx.doi.org/10.1021/ac501423g,2014.
APA	Zhu, Zhi.,Song, Yanling.,Li, Cong.,Zou, Yuan.,Zhu, Ling.,...&杨朝勇.(2014).Monoclonal Surface Display SELEX for Simple, Rapid, Efficient, and Cost-Effective Aptamer Enrichment and Identification.http://dx.doi.org/10.1021/ac501423g.
MLA	Zhu, Zhi,et al."Monoclonal Surface Display SELEX for Simple, Rapid, Efficient, and Cost-Effective Aptamer Enrichment and Identification".http://dx.doi.org/10.1021/ac501423g (2014).