Engineering of Switchable Aptamer Micelle Flares for Molecular Imaging in Living Cells

CORC > 厦门大学 > 化学化工－已发表论文

	Engineering of Switchable Aptamer Micelle Flares for Molecular Imaging in Living Cells
	Wu, Cuichen ; Chen, Tao ; Han, Da ; You, Mingxu ; Peng, Lu ; Cansiz, Sena ; Zhu, Guizhi ; Li, Chunmei ; Xiong, Xiangling ; Jimenez, Elizabeth ; Yang, Chaoyong James ; Tan, Weihong ; Yang CY(杨朝勇)
刊名	http://dx.doi.org/10.1021/nn402517v
	2013
关键词	MESSENGER-RNA DETECTION BREAST-CARCINOMA CELLS NUCLEIC-ACID GOLD NANOPARTICLES NANO-FLARES GENE-REGULATION LIVE CELLS DNA BEACONS PROBE
英文摘要	Society for Analytical Chemists of Pittsburgh; National Institutes of Health [GM066137, GM079359, CA133086]; Simultaneous monitoring of the expression, distribution, and dynamics of biological molecules in living cells is one of the most challenging tasks in the analytical sciences. The key to effective and successful intracellular imaging is the development of delivery platforms with high efficiency and ultrasensitive molecular probes for specific targets of interest. To achieve these goals, many nanomaterials are widely used as carriers to introduce nucleic acid probes into living cells for real-time imaging of biomolecules. However, limitations on their use include issues of cytotoxicity and delivery efficiency. Herein, we propose a switchable aptamer micelle flare (SAME), formed by self-assembly of an aptamer switch probe-diacyllipid chimera, to monitor ATP molecules inside living cells. Similarity of hydrophobic composition between diacyllipids in the micelle flares and phospholipid bilayers in the dynamic membranes of living cells allows SAMFs to be uptaken by living cells more efficiently than aptamer switch probes without external auxiliary. Switchable aptamers were found to bind target ATP molecules with high selectivity and specificity, resulting in restoration of the fluorescence signal from "OFF" to "ON" state, thus indicating the presence of the analyte. These switchable aptamer micelle flares, which exhibit cell permeability and nanoscale controllability, show exceptional promise for molecular imaging in bioanalysis, disease diagnosis, and drug delivery.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88985]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Wu, Cuichen,Chen, Tao,Han, Da,et al. Engineering of Switchable Aptamer Micelle Flares for Molecular Imaging in Living Cells[J]. http://dx.doi.org/10.1021/nn402517v,2013.
APA	Wu, Cuichen.,Chen, Tao.,Han, Da.,You, Mingxu.,Peng, Lu.,...&杨朝勇.(2013).Engineering of Switchable Aptamer Micelle Flares for Molecular Imaging in Living Cells.http://dx.doi.org/10.1021/nn402517v.
MLA	Wu, Cuichen,et al."Engineering of Switchable Aptamer Micelle Flares for Molecular Imaging in Living Cells".http://dx.doi.org/10.1021/nn402517v (2013).