Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis

CORC > 厦门大学 > 化学化工－已发表论文

	Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis
	Cui, Liang ; Ke, Guoliang ; Lin, Xiaoyan ; Song, Yanling ; Zhang, Huimin ; Guan, Zhichao ; Zhu, Zhi ; Yang, Chaoyong James ; Zhu Z(朱志) ; Yang CY(杨朝勇)
刊名	http://dx.doi.org/10.1016/j.ymeth.2013.07.015
	2013
关键词	COMPLEX BIOLOGICAL SAMPLES ACID PHOSPHATASE-EXONUCLEASE SWITCHING SIGNALING APTAMERS MOLECULAR BEACONS DNA DETECTION LABEL-FREE NANOPARTICLE AMPLIFICATION ELECTROCHEMICAL DETECTION RECYCLING AMPLIFICATION NICKING ENDONUCLEASE
英文摘要	National Basic Research Program of China [2010CB732402]; National Science Foundation of China [21205100, 21075104]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]; Nucleic acid molecular probes (NAMPs) have been widely used in the sensing of various chemical and biological substances, as well as physical parameters. However, for traditional nucleic acid molecular probes, the stoichiometric 1:1 binding ratio limits the signal enhancement and thus the sensitivity of the assay. In order to overcome this problem, the cyclic enzymatic amplification method (CEAM) based on exonuclease III has been applied in optical and electrical detection of DNA, proteins and small molecules with excellent sensitivity, selectivity, versatility and simplicity. In this review, the working principle of CEAM is first introduced, followed by the applications of CEAM using different output signals for various analytes. Finally, experimental designs and procedures of CEAM are discussed in detail using displacing probes-based CEAM and linear molecular beacons (LMBs)-based CEAM as two examples. (C) 2013 Elsevier Inc. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88881]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Cui, Liang,Ke, Guoliang,Lin, Xiaoyan,et al. Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis[J]. http://dx.doi.org/10.1016/j.ymeth.2013.07.015,2013.
APA	Cui, Liang.,Ke, Guoliang.,Lin, Xiaoyan.,Song, Yanling.,Zhang, Huimin.,...&杨朝勇.(2013).Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis.http://dx.doi.org/10.1016/j.ymeth.2013.07.015.
MLA	Cui, Liang,et al."Cyclic enzymatic amplification method (CEAM) based on exonuclease III for highly sensitive bioanalysis".http://dx.doi.org/10.1016/j.ymeth.2013.07.015 (2013).