Development and Application of High-throughput and Portable Fluorescence Device

CORC > 厦门大学 > 化学化工－已发表论文

	Development and Application of High-throughput and Portable Fluorescence Device
	Liu Jia-Jia ; Guo Xiang-Qun ; Guo XQ(郭祥群)
刊名	http://dx.doi.org/10.3724/SP.J.1096.2013.30013
	2013
英文摘要	Multichannel and high-throughput fluorescence measuring systems provide a great advantage in speeding up the batch analysis from several-fold to hundred-fold, and would be practically significant in application areas such as hygiene analysis, environmental analysis, food analysis, pharmaceutical analysis, biochemical and clinical analysis. Herein we report the design and fabrication of a novel high-throughput and portable fluorescence device. A line laser pointer was used as the light source, so as to avoid the drawbacks and limitations of conventional complicated optical setup which need professional and exact adjustment. The as-constructed device is simple, portable, low-cost and multichannel. It takes less than 10 min to collect and process data for a 96 microplate-batch analysis of solution samples, by combining use of lab-made software, Chem-Image-Processor (CIP). Its sensitivity was evaluated with Rhodamine B and the limit of detection (LOD) was (2. 80 +/- 0. 15) nmol/L. The new system was further validated by the detection of Hg2+ in aqueous solution and a LOD of 7. 7 mu g/L was obtained. The standard addition recovery of Hg2+ in tap water samples was 99. 9%-102. 4% and the RSD was 1. 5%-2. 6%. The standard addition recovery of Hg2+ in pond water samples was 96. 2%-98. 9 % and the RSD was 1. 6%-2. 5%.
语种	英语
出版者	ELSEVIER SCIENCE INC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88867]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Liu Jia-Jia,Guo Xiang-Qun,Guo XQ. Development and Application of High-throughput and Portable Fluorescence Device[J]. http://dx.doi.org/10.3724/SP.J.1096.2013.30013,2013.
APA	Liu Jia-Jia,Guo Xiang-Qun,&郭祥群.(2013).Development and Application of High-throughput and Portable Fluorescence Device.http://dx.doi.org/10.3724/SP.J.1096.2013.30013.
MLA	Liu Jia-Jia,et al."Development and Application of High-throughput and Portable Fluorescence Device".http://dx.doi.org/10.3724/SP.J.1096.2013.30013 (2013).