Development and Application of High-throughput and Portable Fluorescence Device | |
Liu Jia-Jia ; Guo Xiang-Qun ; Guo XQ(郭祥群) | |
刊名 | http://dx.doi.org/10.3724/SP.J.1096.2013.30013
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2013 | |
英文摘要 | Multichannel and high-throughput fluorescence measuring systems provide a great advantage in speeding up the batch analysis from several-fold to hundred-fold, and would be practically significant in application areas such as hygiene analysis, environmental analysis, food analysis, pharmaceutical analysis, biochemical and clinical analysis. Herein we report the design and fabrication of a novel high-throughput and portable fluorescence device. A line laser pointer was used as the light source, so as to avoid the drawbacks and limitations of conventional complicated optical setup which need professional and exact adjustment. The as-constructed device is simple, portable, low-cost and multichannel. It takes less than 10 min to collect and process data for a 96 microplate-batch analysis of solution samples, by combining use of lab-made software, Chem-Image-Processor (CIP). Its sensitivity was evaluated with Rhodamine B and the limit of detection (LOD) was (2. 80 +/- 0. 15) nmol/L. The new system was further validated by the detection of Hg2+ in aqueous solution and a LOD of 7. 7 mu g/L was obtained. The standard addition recovery of Hg2+ in tap water samples was 99. 9%-102. 4% and the RSD was 1. 5%-2. 6%. The standard addition recovery of Hg2+ in pond water samples was 96. 2%-98. 9 % and the RSD was 1. 6%-2. 5%. |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE INC |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/88867] ![]() |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Liu Jia-Jia,Guo Xiang-Qun,Guo XQ. Development and Application of High-throughput and Portable Fluorescence Device[J]. http://dx.doi.org/10.3724/SP.J.1096.2013.30013,2013. |
APA | Liu Jia-Jia,Guo Xiang-Qun,&郭祥群.(2013).Development and Application of High-throughput and Portable Fluorescence Device.http://dx.doi.org/10.3724/SP.J.1096.2013.30013. |
MLA | Liu Jia-Jia,et al."Development and Application of High-throughput and Portable Fluorescence Device".http://dx.doi.org/10.3724/SP.J.1096.2013.30013 (2013). |
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